Birth of Cloned Microminipigs Derived from Somatic Cell Nuclear Transfer Embryos That Have Been Transiently Treated with Valproic Acid

• Published in: Cellular reprogramming Vol. 18; no. 6; p. 390
• Main Authors: Miyoshi, Kazuchika, Kawaguchi, Hiroaki, Maeda, Kosuke, Sato, Masahiro, Akioka, Kohei, Noguchi, Michiko, Horiuchi, Masahisa, Tanimoto, Akihide
• Format: Journal Article
• Language: English
• Published: United States 01.11.2016
• Subjects: Acetylation; Animals; Blastocyst - cytology; Blastocyst - drug effects; Cloning, Organism - methods; Embryo Transfer - veterinary; Embryo, Mammalian - cytology; Embryo, Mammalian - drug effects; Embryonic Development - drug effects; Enzyme Inhibitors - pharmacology; Female; Histones - metabolism; Nuclear Transfer Techniques - veterinary; Swine; Swine, Miniature; Valproic Acid - pharmacology; reprogramming; somatic cell nuclear transfer; valproic acid; microminipig

In our previous study, we found that treatment of miniature pig somatic cell nuclear transfer (SCNT) embryos with 4 mM valproic acid (VPA), a histone deacetylase inhibitor, for 48 hours after activation enhanced blastocyst formation rate and octamer-binding transcription factor-3/4 (Oct-3/4) gene ex...