Rough microsomes isolated from snap-frozen canine pancreatic tissue retain their co-translational translocation functionality

• Published in: Biology methods and protocols Vol. 10; no. 1; p. bpaf044
• Main Authors: Croonenborghs, Marianne, Verhaegen, Marijke, Pauwels, Eva, Provinciael, Becky, Vermeire, Kurt
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2025
• Subjects: Antiviral agents; CD4 antigen; Cell division; Cell signaling; Endoplasmic reticulum; Eukaryotes; Glycosylation; Immunosuppressive agents; Microsomes; Pancreas; Post-translation; Protein transport; Proteins; Proteomes; Ribosomes; Translation; canine pancreatic microsomes; cell-free in vitro protein translation; Sec61 translocon; co-translational translocation; endoplasmic reticulum; protein translocation

Proteins are essential for life in all organisms: they mediate cell signaling and cell division and provide structure/motility to cells and tissues. All proteins are synthesized on cytoplasmic ribosomes as unfolded precursors that need to find their correct location in the compartmentalized cell. In...