Development of indirect-competitive quartz crystal microbalance immunosensor for C-reactive protein
A highly sensitive indirect-competitive quartz crystal microbalance immunosensor for a cardiovascular biomarker, C-reactive protein (CRP), was developed. The sensor system was constructed with a buffer reservoir, a micro-dispensing pump, an injector, a flow-through cell with a sensor chip, an oscill...
Saved in:
Published in | Sensors and actuators. B, Chemical Vol. 143; no. 1; pp. 444 - 448 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
04.12.2009
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A highly sensitive indirect-competitive quartz crystal microbalance immunosensor for a cardiovascular biomarker, C-reactive protein (CRP), was developed. The sensor system was constructed with a buffer reservoir, a micro-dispensing pump, an injector, a flow-through cell with a sensor chip, an oscillator module, a frequency response analyzer and a PC. The reaction buffer for the system was 0.1
M sodium phosphate buffer (pH 7.0). The optimum concentrations of CRP for immobilization and an anti-CRP antibody for competition reaction were 2 and 0.250
mg/mL, respectively. When plotted in double-logarithmic scale, a linear relationship between CRP concentration and sensor response was obtained in a wide concentration range of 0.130–25,016
ng/mL of CRP, together with the limit of detection of 0.130
ng/mL. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0925-4005 1873-3077 |
DOI: | 10.1016/j.snb.2009.09.046 |