A 20-year journey on the invention of vibrational photothermal microscopy

• Published in: Nature methods Vol. 22; no. 5; pp. 883 - 885
• Main Author: Cheng, Ji-Xin
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.05.2025; Nature Publishing Group
• Subjects: 631/1647/245/2226; 631/57; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Careers; Chemical bonds; Comment; Inventions; Life Sciences; Microscopes; Microscopy; Proteomics; Vibratory communication

Vibrational microscopy opens a new window onto understanding life at the molecular level. Yet the vibrational signals from chemical bonds are weaker than the fluorescence signal from a dye by many orders of magnitude. Detecting such weak signal from a tight focus under a microscope is extremely chal...