Mass spectral characterisation of the major DNA--carcinogen adduct formed from the metabolically activated carcinogen 15,16-dihydro-11-methylcyclopenta[a]phenanthren-17-one

• Published in: Carcinogenesis (New York) Vol. 2; no. 7; p. 637
• Main Authors: Wiebers, J L, Abbott, P J, Coombs, M M, Livingston, D C
• Format: Journal Article
• Language: English
• Published: England 1981
• Subjects: Biotransformation; Carcinogens - analysis; Carcinogens - metabolism; DNA - analysis; DNA - metabolism; Gonanes - analysis; Gonanes - metabolism; Guanine - metabolism; Mass Spectrometry

E. coli DNA, labelled with [14C]adenine and [14C]-guanine, was allowed to react with the [3H]-labelled carcinogen 15,16-dihydro-11-methylcyclopenta[a]phenanthren-17-one in the presence of a microsomal metabolising system. Enzymatic hydrolysis of the DNA followed by Sephadex LH20 chromatography of it...