Electrosynthesis of molecularly imprinted polypyrrole for the antibiotic levofloxacin

The development of an electrosynthesized imprinted polypyrrole (PPY) film onto a platinum sheet as sorbent phase for a fluoroquinolone antibiotic (levofloxacin) is described. Experimental conditions for the electropolymerization of PPY in the presence of the template were optimized. The molecularly...

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Published in	Thin solid films Vol. 520; no. 6; pp. 1938 - 1943
Main Authors	Mazzotta, Elisabetta, Malitesta, Cosimino, Díaz-Álvarez, Myriam, Martin-Esteban, Antonio
Format	Journal Article
Language	English
Published	Amsterdam Elsevier B.V 2012 Elsevier
Subjects	Chemistry Electrochemistry Electropolymerization Exact sciences and technology General and physical chemistry Kinetics and mechanism of reactions Levofloxacin Molecularly imprinted polymer Polypyrrole Levofloxacin Molecularly imprinted polymer Polypyrrole Electropolymerization Solvent effect Template reaction Polymer films Polymer Liquid chromatography Thin film Antibiotic Platinum Imprinting Electrochemistry Pyrrole polymer X-ray photoelectron spectra
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Abstract	The development of an electrosynthesized imprinted polypyrrole (PPY) film onto a platinum sheet as sorbent phase for a fluoroquinolone antibiotic (levofloxacin) is described. Experimental conditions for the electropolymerization of PPY in the presence of the template were optimized. The molecularly imprinted polymer (MIP) film was characterized by X-Ray Photoelectron Spectroscopy (XPS) to verify the template entrapment in the polymeric matrix. After being subject to washing procedures, MIP was analyzed by XPS and a very satisfactory template removal was estimated being equal to 83%. The effectiveness of washing protocol was assessed also by UV–vis and High Performance Liquid Chromatography (HPLC) analysis of corresponding washing solutions. Rebinding experiments were performed by exposing the imprinted PPY film to levofloxacin solutions, subsequently analyzed by HPLC. The effect of solvent and time of exposure was investigated. The imprinting effect was verified by comparing recognition abilities of both MIP and not imprinted polymer (a polymer prepared in the same conditions but in the absence of the template).
AbstractList	The development of an electrosynthesized imprinted polypyrrole (PPY) film onto a platinum sheet as sorbent phase for a fluoroquinolone antibiotic (levofloxacin) is described. Experimental conditions for the electropolymerization of PPY in the presence of the template were optimized. The molecularly imprinted polymer (MIP) film was characterized by X-Ray Photoelectron Spectroscopy (XPS) to verify the template entrapment in the polymeric matrix. After being subject to washing procedures, MIP was analyzed by XPS and a very satisfactory template removal was estimated being equal to 83%. The effectiveness of washing protocol was assessed also by UV–vis and High Performance Liquid Chromatography (HPLC) analysis of corresponding washing solutions. Rebinding experiments were performed by exposing the imprinted PPY film to levofloxacin solutions, subsequently analyzed by HPLC. The effect of solvent and time of exposure was investigated. The imprinting effect was verified by comparing recognition abilities of both MIP and not imprinted polymer (a polymer prepared in the same conditions but in the absence of the template).
Author	Martin-Esteban, Antonio Mazzotta, Elisabetta Malitesta, Cosimino Díaz-Álvarez, Myriam
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Keywords	Levofloxacin Molecularly imprinted polymer Polypyrrole Electropolymerization Solvent effect Template reaction Polymer films Polymer Liquid chromatography Thin film Antibiotic Platinum Imprinting Electrochemistry Pyrrole polymer X-ray photoelectron spectra
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Snippet	The development of an electrosynthesized imprinted polypyrrole (PPY) film onto a platinum sheet as sorbent phase for a fluoroquinolone antibiotic...
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SubjectTerms	Chemistry Electrochemistry Electropolymerization Exact sciences and technology General and physical chemistry Kinetics and mechanism of reactions Levofloxacin Molecularly imprinted polymer Polypyrrole
Title	Electrosynthesis of molecularly imprinted polypyrrole for the antibiotic levofloxacin
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