Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney

• Published in: Amino acids Vol. 30; no. 4; pp. 495 - 498
• Main Authors: Simic, T, Pljesa-Ercegovac, M, Savic-Radojevic, A, Hadziahmetovic, M, Mimic-Oka, J
• Format: Journal Article
• Language: English
• Published: Austria Springer Nature B.V 01.06.2006
• Subjects: Chemical Phenomena; Chemistry, Physical; Chromatography, Affinity - methods; Chromatography, Agarose - methods; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors - pharmacology; Enzymes; Ethacrynic Acid - pharmacology; Glutathione - chemistry; Glutathione Transferase - antagonists & inhibitors; Glutathione Transferase - chemistry; Glutathione Transferase - isolation & purification; Hemin - pharmacology; Humans; Immunoblotting; Kidney - enzymology; Sensitivity and Specificity; Triazines - pharmacology

To identify kidney glutathione S-transferase (GST) isoenzyme, which does not bind to glutathione affinity column, biochemical characterization was performed by using an array of substrates and by measuring sensitivity to inhibitors. Immunological characterization was done by immunoblotting. Affinity...