Selective Promoter Usage of the Human Estrogen Receptor-α Gene and Its Regulation by Estrogen

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 13; no. 11; pp. 1934 - 1950
• Main Authors: Donaghue, C, Westley, B. R, May, F. E. B
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01.11.1999
• Subjects: Breast Neoplasms - genetics; Carcinoma - genetics; Cloning, Molecular; Estrogen Receptor alpha; Estrogens - metabolism; Gene Expression Regulation; Genes, Reporter; HeLa Cells; Humans; Promoter Regions, Genetic; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Response Elements - genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Tumor Cells, Cultured

Three promoters have been identified for the human estrogen receptor-α gene. The positions of promoters A and B are known whereas that of the recently identified promoter C is not. Cloning and hybridization experiments demonstrated that promoter C is located more than 21 kb upstream of promoter A. T...