Techniques for Selective Labeling of Molecules and Subcellular Structures for Cryo-Electron Tomography

• Published in: Biochemistry (Moscow) Vol. 90; no. 2; pp. 173 - 187
• Main Authors: Kazakov, Evgeny P., Kireev, Igor I., Golyshev, Sergei A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.02.2025; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Cellular structure; Cryoelectron Microscopy - methods; Crystallization; Dehydration; Electron Microscope Tomography - methods; Electron microscopy; Fine structure; Freezing; Humans; Labeling; Life Sciences; Light microscopy; Localization; Microbiology; Microscopy; Optical microscopy; Proteins; Proteins - chemistry; Review; Staining and Labeling - methods; Tomography; Ultrastructure; quantum dots; encapsulins; ferritin; gold nanoparticles; correlation microscopy; electron microscopy; cryo-electron tomography; metallothionein; genetically encoded tags

Electron microscopy (EM) is one of the most efficient methods for studying the fine structure of cells with a resolution thousands of times higher than that of visible light microscopy. The most advanced implementation of electron microscopy in biology is EM tomography of samples stabilized by freez...