Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae

The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae , thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-base...

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Published inBrazilian journal of microbiology Vol. 54; no. 3; pp. 1819 - 1825
Main Authors Dadwal, Rajneesh, Paul, Saikat, Gupta, Parakriti, Yadav, Rakesh, Sood, Seema, Ghosh, A. K., Shivaprakash, M. R., Gainder, Shalini, Sethi, Sunil
Format Journal Article
LanguageEnglish
Published Cham Springer International Publishing 01.09.2023
Springer Nature B.V
Subjects
Anti-Bacterial Agents - pharmacology
Antibiotics
Azithromycin
Biomedical and Life Sciences
Ciprofloxacin
Ciprofloxacin - pharmacology
Clinical Microbiology - Research Paper
Culture Media, Conditioned
Drug resistance
Food Microbiology
Ionization
Isotope Labeling
Labeling
Life Sciences
Lysine
Mass spectrometry
Mass spectroscopy
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbial Sensitivity Tests
Microbiology
Mycobacterium tuberculosis
Mycology
Neisseria gonorrhoeae
Spectra
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Stable isotopes
Sexually transmitted infection
SILAC
MALDI-TOF
Ciprofloxacin
Resistance detection
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Abstract The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae , thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae . All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.
AbstractList The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae , thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae . All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.
The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae, thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae. All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.
Author Yadav, Rakesh
Sethi, Sunil
Gainder, Shalini
Dadwal, Rajneesh
Ghosh, A. K.
Shivaprakash, M. R.
Gupta, Parakriti
Paul, Saikat
Sood, Seema
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  email: sunilsethi10@gmail.com, sunilsethi10@hotmail.com
  organization: Department of Medical Microbiology, PGIMER
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Keywords Sexually transmitted infection
SILAC
MALDI-TOF
Ciprofloxacin
Resistance detection
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Snippet The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae , thereby underlining the significance of rapid and accurate...
The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae, thereby underlining the significance of rapid and accurate...
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SubjectTerms Anti-Bacterial Agents - pharmacology
Antibiotics
Azithromycin
Biomedical and Life Sciences
Ciprofloxacin
Ciprofloxacin - pharmacology
Clinical Microbiology - Research Paper
Culture Media, Conditioned
Drug resistance
Food Microbiology
Ionization
Isotope Labeling
Labeling
Life Sciences
Lysine
Mass spectrometry
Mass spectroscopy
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbial Sensitivity Tests
Microbiology
Mycobacterium tuberculosis
Mycology
Neisseria gonorrhoeae
Spectra
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Stable isotopes
Title Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae
URI https://link.springer.com/article/10.1007/s42770-023-00996-2
https://www.ncbi.nlm.nih.gov/pubmed/37258877
https://www.proquest.com/docview/2862589459
https://www.proquest.com/docview/2821638896
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