Exosomes from Bone Marrow Dendritic Cells Pulsed with Diphtheria Toxoid Preferentially Induce Type 1 Antigen-Specific IgG Responses in Naive Recipients in the Absence of Free Antigen

• Published in: Journal of Immunology Vol. 177; no. 6; pp. 3757 - 3762
• Main Authors: Colino, Jesus, Snapper, Clifford M
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.09.2006
• Subjects: Animals; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - classification; Bone Marrow Cells - cytology; Bone Marrow Cells - immunology; Bone Marrow Cells - microbiology; Cattle; Cell Polarity - immunology; Cells, Cultured; Dendritic Cells - cytology; Dendritic Cells - immunology; Dendritic Cells - microbiology; Diphtheria Toxoid - immunology; Diphtheria Toxoid - metabolism; Drug Contamination; Epitopes - biosynthesis; Female; Immunoglobulin G - biosynthesis; Immunoglobulin G - classification; Immunoglobulin Isotypes - biosynthesis; Interleukin-12 - analysis; Interleukin-6 - analysis; Mice; Mice, Inbred BALB C; Transport Vesicles - immunology; Transport Vesicles - metabolism; Transport Vesicles - microbiology

Exosomes derived from dendritic cells (DC) activate T cells in vivo, but whether exosomes are able to induce and/or modulate humoral immune responses is still unknown. We show that murine bone marrow DC pulsed in vitro with an intact protein (diphtheria toxoid (DT)) produce exosomes that induce, in...