The alternative sigma factor σ X mediates competence shut-off at the cell pole in Streptococcus pneumoniae

Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal localization dynamics of the key regulators that master the two intertwined and transient transcription waves defining competence in . The f...

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Published ineLife Vol. 9
Main Authors Johnston, Calum Hg, Soulet, Anne-Lise, Bergé, Matthieu, Prudhomme, Marc, De Lemos, David, Polard, Patrice
Format Journal Article
LanguageEnglish
Published England eLife Sciences Publication 02.11.2020
eLife Sciences Publications, Ltd
eLife Sciences Publications Ltd
Subjects
alternative sigma factor
bacterial competence regulation
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biochemistry, Molecular Biology
Gene Expression Regulation, Bacterial - physiology
genetic regulatory networks
Genomics
Life Sciences
Membrane Proteins - genetics
Membrane Proteins - metabolism
Microbiology and Infectious Disease
Microbiology and Parasitology
Microscopy, Fluorescence
polar competence hub
Sigma Factor - genetics
Sigma Factor - metabolism
streptococcus pneumoniae
Streptococcus pneumoniae - cytology
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - metabolism
Transformation, Genetic
alternative sigma factor
genetic regulatory networks
infectious disease
microbiology
streptococcus pneumoniae
bacterial competence regulation
polar competence hub
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Abstract Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal localization dynamics of the key regulators that master the two intertwined and transient transcription waves defining competence in . The first wave relies on the stress-inducible phosphorelay between ComD and ComE proteins, and the second on the alternative sigma factor σ , which directs the expression of the DprA protein that turns off competence through interaction with phosphorylated ComE. We found that ComD, σ and DprA stably co-localize at one pole in competent cells, with σ physically conveying DprA next to ComD. Through this polar DprA targeting function, σ mediates the timely shut-off of the pneumococcal competence cycle, preserving cell fitness. Altogether, this study unveils an unprecedented role for a transcription σ factor in spatially coordinating the negative feedback loop of its own genetic circuit.
AbstractList Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal localization dynamics of the key regulators that master the two intertwined and transient transcription waves defining competence in Streptococcus pneumoniae . The first wave relies on the stress-inducible phosphorelay between ComD and ComE proteins, and the second on the alternative sigma factor σ X , which directs the expression of the DprA protein that turns off competence through interaction with phosphorylated ComE. We found that ComD, σ X and DprA stably co-localize at one pole in competent cells, with σ X physically conveying DprA next to ComD. Through this polar DprA targeting function, σ X mediates the timely shut-off of the pneumococcal competence cycle, preserving cell fitness. Altogether, this study unveils an unprecedented role for a transcription σ factor in spatially coordinating the negative feedback loop of its own genetic circuit.
Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal localization dynamics of the key regulators that master the two intertwined and transient transcription waves defining competence in . The first wave relies on the stress-inducible phosphorelay between ComD and ComE proteins, and the second on the alternative sigma factor σ , which directs the expression of the DprA protein that turns off competence through interaction with phosphorylated ComE. We found that ComD, σ and DprA stably co-localize at one pole in competent cells, with σ physically conveying DprA next to ComD. Through this polar DprA targeting function, σ mediates the timely shut-off of the pneumococcal competence cycle, preserving cell fitness. Altogether, this study unveils an unprecedented role for a transcription σ factor in spatially coordinating the negative feedback loop of its own genetic circuit.
Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal localization dynamics of the key regulators that master the two intertwined and transient transcription waves defining competence in Streptococcus pneumoniae. The first wave relies on the stress-inducible phosphorelay between ComD and ComE proteins, and the second on the alternative sigma factor σX, which directs the expression of the DprA protein that turns off competence through interaction with phosphorylated ComE. We found that ComD, σX and DprA stably co-localize at one pole in competent cells, with σX physically conveying DprA next to ComD. Through this polar DprA targeting function, σX mediates the timely shut-off of the pneumococcal competence cycle, preserving cell fitness. Altogether, this study unveils an unprecedented role for a transcription σ factor in spatially coordinating the negative feedback loop of its own genetic circuit.
Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal localization dynamics of the key regulators that master the two intertwined and transient transcription waves defining competence in Streptococcus pneumoniae. The first wave relies on the stress-inducible phosphorelay between ComD and ComE proteins, and the second on the alternative sigma factor s X , which directs the expression of the DprA protein that turns off competence through interaction with phosphorylated ComE. We found that ComD, s X and DprA stably co-localize at one pole in competent cells, with s X physically conveying DprA next to ComD. Through this polar DprA targeting function, s X mediates the timely shut-off of the pneumococcal competence cycle, preserving cell fitness. Altogether, this study unveils an unprecedented role for a transcription s factor in spatially coordinating the negative feedback loop of its own genetic circuit.
Author Polard, Patrice
De Lemos, David
Soulet, Anne-Lise
Johnston, Calum Hg
Prudhomme, Marc
Bergé, Matthieu
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Keywords alternative sigma factor
genetic regulatory networks
infectious disease
microbiology
streptococcus pneumoniae
bacterial competence regulation
polar competence hub
Language English
License 2020, Johnston et al.
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Snippet Competence is a widespread bacterial differentiation program driving antibiotic resistance and virulence in many pathogens. Here, we studied the spatiotemporal...
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SubjectTerms alternative sigma factor
bacterial competence regulation
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biochemistry, Molecular Biology
Gene Expression Regulation, Bacterial - physiology
genetic regulatory networks
Genomics
Life Sciences
Membrane Proteins - genetics
Membrane Proteins - metabolism
Microbiology and Infectious Disease
Microbiology and Parasitology
Microscopy, Fluorescence
polar competence hub
Sigma Factor - genetics
Sigma Factor - metabolism
streptococcus pneumoniae
Streptococcus pneumoniae - cytology
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - metabolism
Transformation, Genetic
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Title The alternative sigma factor σ X mediates competence shut-off at the cell pole in Streptococcus pneumoniae
URI https://www.ncbi.nlm.nih.gov/pubmed/33135635
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https://hal.science/hal-03411156
https://pubmed.ncbi.nlm.nih.gov/PMC7665891
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Volume 9
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