A capillary column packed with a zirconium(IV)-based organic framework for enrichment of endogenous phosphopeptides

• Published in: Mikrochimica acta (1966) Vol. 185; no. 12; pp. 562 - 8
• Main Authors: Lin, Haizhu, Chen, Hemei, Shao, Xi, Deng, Chunhui
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.12.2018; Springer; Springer Nature B.V
• Subjects: Analysis; Analytical Chemistry; Casein; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Ionization; Ions; Mass spectrometry; Metal-organic frameworks; Microengineering; Microspheres; Nanochemistry; Nanotechnology; Original Paper; Phosphates; Post-translational modification; Saliva; Selectivity; Silicon dioxide; Zirconium; MALDI-TOF MS; MOF; Human saliva; Phosphopeptide; Polydopamine; Metal oxide affinity chromatography; Phosphoproteome; Size exclusion effect; Mass spectrometry; On-column enrichment

A zirconium(IV)-based metal organic framework (Zr-MOF) was deposited on polydopamine-coated silica microspheres to form microspheres of type SiO 2 @PDA@Zr-MOF. These were packed into capillary columns for enrichment of phosphopeptides. The column was off-line coupled to both matrix-assisted laser de...