In situ synthesis of a novel metal oxide affinity chromatography affinity probe for the selective enrichment of low‐abundance phosphopeptides

• Published in: Rapid communications in mass spectrometry Vol. 34; no. 20; pp. e8881 - n/a
• Main Authors: Wang, Baichun, Wu, Huanming, Yan, Yinghua, Tang, Keqi, Ding, Chuan‐Fan
• Format: Journal Article
• Language: English
• Published: Bognor Regis Wiley Subscription Services, Inc 30.10.2020
• Subjects: Affinity; Biological properties; Casein; Chromatography; Enrichment; Graphene; Lewis acid; Mass spectrometry; Metal oxides; Phosphorylation; Recyclability; Selectivity; Serum albumin; Stoichiometry; Zirconium dioxide

Rationale Due to the dynamic nature of phosphorylation states and the low stoichiometry of phosphopeptides, it is still a challenge to efficiently capture phosphopeptides from complex biological samples before mass spectrometry analysis. Among the enrichment strategies, metal oxide affinity chromato...