Evaluation of unexpected protecting group removal in solid‐phase peptide synthesis: Quantified using continuous flow methods

• Published in: Journal of peptide science Vol. 28; no. 12; pp. e3441 - n/a
• Main Authors: Laude, Victoire, Nuño, Manuel, Moses, Roger C., Guthrie, Duncan
• Format: Journal Article
• Language: English
• Published: Bognor Regis Wiley Subscription Services, Inc 01.12.2022
• Subjects: Additives; CF‐SPPS; Continuous flow; Dimethylformamide; Fmoc removal; Fmoc stability; Impurities; in‐line analysis; Peptide synthesis; Peptides; Protecting group removal; Solvents; SPPS; Synthesis

As peptides gained interest as new drugs in the past years, their synthetic routes had been the subject of review and improvement. Fmoc/tBu‐based solid‐phase peptide synthesis (SPPS) is the most convenient technique, and the implementation in continuous flow has allowed for single‐pass coupling and...