Quantification of PKC family genes in sporadic breast cancer by qRT-PCR: Evidence that PKCι/λ overexpression is an independent prognostic factor

• Published in: International journal of cancer Vol. 131; no. 12; pp. 2852 - 2862
• Main Authors: Awadelkarim, Khalid Dafaallah, Callens, Céline, Rossé, Carine, Susini, Aurélie, Vacher, Sophie, Rouleau, Etienne, Lidereau, Rosette, Bièche, Ivan
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.12.2012
• Subjects: Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Female; Gene Expression Regulation, Enzymologic; Humans; Multigene Family; PKC family gene expression; Prognosis; prognostic significance; Protein Kinase C - genetics; quantitative real-time RT-PCR; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics

Drugs targeting protein kinase C (PKC) show promising therapeutic activity. However, little is known about the expression patterns of the 11 PKC genes in human tumors, and the clinical significance of most PKC genes is unknown. We used qRT‐PCR assays to quantify mRNA levels of the 11 PKC genes in 45...