Prolonged interval between fusion and activation impairs embryonic development by inducing chromosome scattering and nuclear aneuploidy in pig somatic cell nuclear transfer

• Published in: Reproduction fertility and development Vol. 22; no. 6; pp. 977 - 986
• Main Authors: You, Jinyoung, Song, Kilyoung, Lee, Eunsong
• Format: Journal Article
• Language: English
• Published: Australia Collingwood, Victoria: CSIRO Publishing 2010
• Subjects: Aneuploidy; Animals; Cells, Cultured; Embryo Culture Techniques; Embryonic Development - genetics; maturation-promoting factor; nuclear ploidy; nuclear remodelling; Nuclear Transfer Techniques; Oocytes - physiology; pseudopronucleus; Swine; Time Factors

The aim of the present study was to examine the effect of various intervals between electrofusion and activation (FA interval) on the nuclear remodelling and development of somatic cell nuclear transfer (SCNT) embryos in pigs. Reconstructed oocytes were activated at 0 (simultaneous fusion and activa...