Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy

The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in...

Full description

Saved in:
Bibliographic Details
Published inBiocell Vol. 45; no. 1; pp. 189 - 198
Main Authors QIAN, YAO, WANG, JINGPING, JI, ZIWAN, CHEN, HAO, HE, YUCANG, LV, XULING, ZHANG, ZIKAI, LI, TIAN, PAN, TIANYUN, LI, LIQUN, LIN, MING
Format Journal Article
LanguageEnglish
Published Mendoza Tech Science Press 2021
Subjects
Acetyl-L-carnitine
Autophagy
Carnitine
Hydrogen peroxide
Intracellular
Oxidative stress
Phagocytosis
Reactive oxygen species
Stem cells
Online AccessGet full text

Cover

More Information
Summary:The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H2O2 (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.
ISSN:1667-5746
0327-9545
DOI:10.32604/biocell.2021.011827