Chemoreceptors from the commensal gut Roseburia rectibacter bind to mucin and trigger chemotaxis

• Published in: Environmental microbiology Vol. 25; no. 7; pp. 1329 - 1343
• Main Authors: Chen, Hong‐He, Wang, Yu‐Xin, Li, De‐Feng, Liu, Chang, Bi, Shuang‐Yu, Jiang, Cheng‐Ying, Liu, Shuang‐Jiang
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.07.2023; Wiley Subscription Services, Inc
• Subjects: Bacteria; Bacteria - metabolism; Bacterial Proteins - metabolism; Chemoreceptors; Chemotaxis; Colonization; Comamonas testosteroni; Digestive system; Digestive tract; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Flagella; Gastrointestinal system; Gastrointestinal tract; Genes; Genomes; Ligands; Microorganisms; Mucin; Mucins - metabolism; Pathogens; Propionates - metabolism; Propionic acid; Roseburia rectibacter; Virulence
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.16365

Chemotaxis is crucial for bacterial adherence and colonization of the host gastrointestinal tract. Previous studies have demonstrated that chemotaxis affects the virulence of causative pathogens and the infection in the host. However, the chemotactic abilities of non‐pathogenic and commensal gut bacteria have rarely been explored. We observed that Roseburia rectibacter NSJ‐69 exhibited flagella‐dependent motility and chemotaxis to a variety of molecules, including mucin and propionate. A genome‐wide analysis revealed that NSJ‐69 has 28 putative chemoreceptors, 15 of which have periplasmic ligand‐binding domains (LBDs). These LBD‐coding genes were chemically synthesized and expressed heterologously in Escherichia coli. Intensive screening of ligands revealed four chemoreceptors bound to mucin and two bound to propionate. When expressed in Comamonas testosteroni or E. coli, these chemoreceptors elicited chemotaxis toward mucin and propionate. Hybrid chemoreceptors were constructed, and results showed that the chemotactic responses to mucin and propionate were dependent on the LBDs of R. rectibacter chemoreceptors. Our study identified and characterized R. rectibacter chemoreceptors. These results will facilitate further investigations on the involvement of microbial chemotaxis in host colonization.