Repair of 8-methoxypsoralen photoinduced cross-links in yeast. Analysis by alkaline step-elution and electron microscopy

• Published in: Molecular & general genetics Vol. 228; no. 3; p. 335
• Main Authors: Cundari, E, Dardalhon, M, Rousset, S, Averbeck, D
• Format: Journal Article
• Language: English
• Published: Germany 01.09.1991
• Subjects: adn; Cross-Linking Reagents; dna; DNA Repair; DNA, Fungal - genetics; DNA, Fungal - ultrastructure; electrical engineering; Fungal Proteins - genetics; genie electrique; Hydrogen-Ion Concentration; immobilisation; immobilization; ingenieria electrica; inmovilizacion; light; lumiere; luz; Methoxsalen - toxicity; Microscopy, Electron; saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Ultraviolet Rays

The repair of interstrand cross-links induced by 8-methoxypsoralen plus UVA (365 nm) radiation DNA was analyzed in diploid strains of the yeast Saccharomyces cerevisiae. The strains employed were the wild-type D7 and derivatives homozygous for the rad18-1 or the rad3-12 mutation. Alkaline step-eluti...