Lipid Peroxidation, Protein Oxidation, Gelatinases, and Their Inhibitors in a Group of Adults with Obesity

• Published in: Hormone and metabolic research Vol. 51; no. 6; p. 389
• Main Authors: Caimi, Gregorio, Canino, Baldassare, Montana, Maria, Urso, Caterina, Calandrino, Vincenzo, Presti, Rosalia Lo, Hopps, Eugenia
• Format: Journal Article
• Language: English
• Published: Germany 01.06.2019
• Subjects: Adult; Biomarkers - analysis; Case-Control Studies; Female; Humans; Lipid Peroxidation; Male; Matrix Metalloproteinase 2 - blood; Matrix Metalloproteinase 9 - blood; Middle Aged; Obesity - blood; Obesity - physiopathology; Oxidation-Reduction; Oxidative Stress; Proteins - chemistry; Proteolysis; Tissue Inhibitor of Metalloproteinase-1 - blood; Tissue Inhibitor of Metalloproteinase-2 - blood
• DOI: 10.1055/a-0887-2770

The association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30-35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.