Synthesis of (R)-2-phenylpropanoic acid from its racemate through an isomerase-involving reaction by Nocardia diaphanozonaria

• Published in: Biotechnology letters Vol. 24; no. 19; pp. 1615 - 1621
• Main Authors: MITSUKURA, Koichi, YOSHIDA, Toyokazu, NAGASAWA, Toru
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.10.2002; Springer Nature B.V
• Subjects: Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Metabolism. Enzymes; Microbiology; inducer; Nocardia; Isomerization; Propionic acid; reaction mechanisms; Nocardiaceae; Biosynthesis; 2-phenylpropanoic acid; Actinomycetales; Substrate; Racemic; Substrate specificity; Microorganism culture; Nocardia diaphanozonaria; Bacteria; Actinomycetes

(R)-2-Phenylpropanoic acid was synthesized from the racemic acid through an isomerization reaction involving resting cells of Nocardia diaphanozonaria JCM3208. The isomerization activity of the cells was enhanced 25-fold by adding 5.5 mM racemic 2-phenylpropanoic acid to the culture medium. When 5 m...