High-throughput quantitative and qualitative analysis of microsomal incubations by cocktail analysis with an ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometer system

• Published in: Bioanalysis Vol. 7; no. 6; pp. 671 - 683
• Main Authors: Backfisch, Gisela, Reder-Hilz, Beate, Hoeckels-Messemer, Juliane, Angstenberger, Jonas, Sydor, Jens, Laplanche, Loic, Schulz, Michael
• Format: Journal Article
• Language: English
• Published: England 01.01.2015
• Subjects: Animals; Chromatography, High Pressure Liquid; Humans; Mass Spectrometry; Metabolomics - methods; Microsomes, Liver - metabolism; Rats; Software

Metabolite identification studies are very resource intensive and also are rarely performed in early discovery. Here, we report the validation of an ultraperformance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS) platform for generating high-throughput stability data with struct...