Caspase Sensors Based on NanoLuc

• Published in: Journal of biotechnology Vol. 357; pp. 100 - 107
• Main Authors: Li, Jie, Wang, Jin-lan, Gan, Chun-Yang, Cai, Xue-fei, Wang, Yu-wei, Long, Quan-xin, Sun, Yu-xue, Wei, Xia-fei, Cui, Jing, Huang, Ai-Long, Hu, Jie-Li
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 20.09.2022
• Subjects: biosensors; biotechnology; caspase; caspase-1; caspase-3; detection; inflammation; luciferase; method; NanoLuc; peptides; programmed cell death; sensor; signal-to-noise ratio; Tobacco etch virus; detection; sensor; NanoLuc; method; caspase

Caspases are a family of evolutionary conserved cysteine proteases that play key roles in programmed cell death and inflammation. Among the methods for the detection of caspase activity, biosensors based on luciferases have advantages in genetical encoding and convenience in assay. In this study, we...