Relationship Between Apoptosis Induction in Cancer Cells and the Amount of Electrical Stimulation on Frequency Analysis of Nanosecond Pulsed Electric Field

ABSTRACT Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects. However, the relationship between the electrical stress induced in cells by the external electric field and the biological response has not be...

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Published inElectrical engineering in Japan Vol. 218; no. 1
Main Authors Ninagawa, Yuto, Sugiura, Ren, Kato, Eiko, Yagi, Ippei, Nagata, Kohki, Yamaoka, Hidehiko, Tachibana, Kosuke, Oda, Akinori, Uchida, Satoshi
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc 01.03.2025
Subjects
Apoptosis
Cancer
cancer cell
Electric fields
Electrical engineering
energy consumption
Equivalent circuits
Frequency analysis
nanosecond pulsed electric field
Nanosecond pulses
pulse width
Side effects
Stimulation
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Abstract ABSTRACT Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects. However, the relationship between the electrical stress induced in cells by the external electric field and the biological response has not been fully investigated quantitatively. We investigated the effects of nsPEF therapy on cancer cells using microgap electrodes under low‐voltage conditions. We developed an equivalent circuit model using intracellular components to determine the amount of stimulation of cellular components and performed frequency analysis of nsPEF. nsPEF applied to Jurkat cells activated caspase‐3 and induced apoptosis at shorter pulse widths. nsPEF induced biological responses and apoptosis in cancer cells. The relationship between the biological response induced by nsPEF and the amount of electrical stimulation was investigated by comparing the experimental results with frequency analysis. Using quantitative values for each cellular element, the relationship between the biological response and the amount of electrical stimulation can be integrated.
AbstractList Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects. However, the relationship between the electrical stress induced in cells by the external electric field and the biological response has not been fully investigated quantitatively. We investigated the effects of nsPEF therapy on cancer cells using microgap electrodes under low‐voltage conditions. We developed an equivalent circuit model using intracellular components to determine the amount of stimulation of cellular components and performed frequency analysis of nsPEF. nsPEF applied to Jurkat cells activated caspase‐3 and induced apoptosis at shorter pulse widths. nsPEF induced biological responses and apoptosis in cancer cells. The relationship between the biological response induced by nsPEF and the amount of electrical stimulation was investigated by comparing the experimental results with frequency analysis. Using quantitative values for each cellular element, the relationship between the biological response and the amount of electrical stimulation can be integrated.
ABSTRACT Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects. However, the relationship between the electrical stress induced in cells by the external electric field and the biological response has not been fully investigated quantitatively. We investigated the effects of nsPEF therapy on cancer cells using microgap electrodes under low‐voltage conditions. We developed an equivalent circuit model using intracellular components to determine the amount of stimulation of cellular components and performed frequency analysis of nsPEF. nsPEF applied to Jurkat cells activated caspase‐3 and induced apoptosis at shorter pulse widths. nsPEF induced biological responses and apoptosis in cancer cells. The relationship between the biological response induced by nsPEF and the amount of electrical stimulation was investigated by comparing the experimental results with frequency analysis. Using quantitative values for each cellular element, the relationship between the biological response and the amount of electrical stimulation can be integrated.
Author Uchida, Satoshi
Yamaoka, Hidehiko
Sugiura, Ren
Kato, Eiko
Oda, Akinori
Nagata, Kohki
Ninagawa, Yuto
Yagi, Ippei
Tachibana, Kosuke
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10.1541/ieejfms.144.407
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Snippet ABSTRACT Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects....
Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects. However,...
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SubjectTerms Apoptosis
Cancer
cancer cell
Electric fields
Electrical engineering
energy consumption
Equivalent circuits
Frequency analysis
nanosecond pulsed electric field
Nanosecond pulses
pulse width
Side effects
Stimulation
Title Relationship Between Apoptosis Induction in Cancer Cells and the Amount of Electrical Stimulation on Frequency Analysis of Nanosecond Pulsed Electric Field
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