A metabolite of an antineoplastic ether phospholipid may inhibit transmembrane signalling via protein kinase C
In our search for the mechanisms by which the drug 1‐O‐alkyl‐2‐O‐methylglycero‐3‐phosphocholine (AMG‐PC) inhibits tumor growth and metastasis, we have detected a metabolite, 1‐O‐alkyl‐2‐O‐methylglycerol (AMG), in membranes of MO4 mouse fibrosarcoma cells grown in the presence of the drug. Synthetic...
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Published in | Lipids Vol. 22; no. 11; pp. 842 - 846 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer‐Verlag
01.11.1987
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Subjects | |
Online Access | Get full text |
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Summary: | In our search for the mechanisms by which the drug 1‐O‐alkyl‐2‐O‐methylglycero‐3‐phosphocholine (AMG‐PC) inhibits tumor growth and metastasis, we have detected a metabolite, 1‐O‐alkyl‐2‐O‐methylglycerol (AMG), in membranes of MO4 mouse fibrosarcoma cells grown in the presence of the drug. Synthetic AMG inhibited the activation of highly purified human protein kinase C by diacylglycerol in the presence of phosphatidylserine. Furthermore, AMG also inhibited the receptor‐specific binding of3H‐phorbol‐12,13‐dibutyrate to human HL‐60 promyeloid leukemia cells in a dose‐dependent fashion. AMG‐PC was not effective or much less so in these assays. We suggest that interaction of the metabolite AMG with protein kinase C may inhibit stimulus‐response coupling in tumor cells and may thus potentially contribute to the mechanism by which AMG‐PC exerts its anticancer activities. |
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Bibliography: | Presented at the symposium on “Ether Lipids in Oncology,” Göttingen, Federal Republic of Germany, December, 1986. |
ISSN: | 0024-4201 1558-9307 |
DOI: | 10.1007/BF02535541 |