Thrombin Receptor Activation Inhibits Monocyte Spreading by Induction of ETB Receptor-Coupled Nitric Oxide Release
Abstract The effect of thrombin receptor activation on monocyte conformation was evaluated using the human monocyte cell line, THP-1, and the thrombin mimetic peptide, Trap-14. Treatment of THP-1 cells with Trap-14 induced rapid rounding of ameboid cells adherent to fibronectin-coated slides, wherea...
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Published in | The Journal of immunology (1950) Vol. 161; no. 9; pp. 5039 - 5044 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Am Assoc Immnol
01.11.1998
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Online Access | Get full text |
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Abstract | Abstract
The effect of thrombin receptor activation on monocyte conformation was evaluated using the human monocyte cell line, THP-1, and the thrombin mimetic peptide, Trap-14. Treatment of THP-1 cells with Trap-14 induced rapid rounding of ameboid cells adherent to fibronectin-coated slides, whereas cell rounding was abrogated in the presence of the nitric oxide synthase inhibitor, NG-nitro-l-arginine or the endothelin B receptor antagonist, BQ-788. Endothelin-1 (ET-1) levels in the culture supernatant increased markedly within minutes of Trap-14 exposure with a concomitant loss in cellular ET-1 immunoreactivity. Importantly, loss of ET-1 immunoreactivity was blocked by pretreatment with the vesicle translocation inhibitor, nocodazole. Trap-14 potently induced the release of NO from THP-1 cells, whereas NO release was ablated by preincubation with BQ-788. These data demonstrate that thrombin receptor activation may inhibit cellular spreading as a result of autocrine ET-1 release and subsequent endothelin B receptor-dependent NO production, and suggest that initial exposure of inflammatory cells to thrombin may limit cellular activation and recruitment. |
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AbstractList | Abstract
The effect of thrombin receptor activation on monocyte conformation was evaluated using the human monocyte cell line, THP-1, and the thrombin mimetic peptide, Trap-14. Treatment of THP-1 cells with Trap-14 induced rapid rounding of ameboid cells adherent to fibronectin-coated slides, whereas cell rounding was abrogated in the presence of the nitric oxide synthase inhibitor, NG-nitro-l-arginine or the endothelin B receptor antagonist, BQ-788. Endothelin-1 (ET-1) levels in the culture supernatant increased markedly within minutes of Trap-14 exposure with a concomitant loss in cellular ET-1 immunoreactivity. Importantly, loss of ET-1 immunoreactivity was blocked by pretreatment with the vesicle translocation inhibitor, nocodazole. Trap-14 potently induced the release of NO from THP-1 cells, whereas NO release was ablated by preincubation with BQ-788. These data demonstrate that thrombin receptor activation may inhibit cellular spreading as a result of autocrine ET-1 release and subsequent endothelin B receptor-dependent NO production, and suggest that initial exposure of inflammatory cells to thrombin may limit cellular activation and recruitment. |
Author | Magazine, Harold I Srivastava, Kamal D |
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CitedBy_id | crossref_primary_10_1161_CIRCULATIONAHA_109_849539 crossref_primary_10_4049_jimmunol_165_1_102 crossref_primary_10_1177_147323000503300510 crossref_primary_10_1111_j_1471_4159_2009_06175_x crossref_primary_10_1002_jnr_10847 crossref_primary_10_1371_journal_pone_0015775 crossref_primary_10_1016_j_freeradbiomed_2009_06_006 crossref_primary_10_1038_s41467_021_27406_1 crossref_primary_10_1111_j_1537_2995_2006_00861_x crossref_primary_10_1016_S1054_8807_00_00045_4 crossref_primary_10_1111_j_1748_1716_2009_02037_x crossref_primary_10_1161_CIRCULATIONAHA_109_889535 |
Cites_doi | 10.1006/bbrc.1993.1294 10.4049/jimmunol.156.12.4845 10.1002/jcp.1041280318 10.1182/blood.V80.8.1989.1989 10.4049/jimmunol.158.2.880 10.1073/pnas.93.13.6448 10.1152/jappl.1995.78.5.1725 10.4049/jimmunol.150.10.4601 10.1042/bj2860945 10.1152/ajpcell.1996.271.3.C923 10.1016/0960-5428(95)00030-5 10.1073/pnas.91.24.11728 10.1096/fasebj.7.13.8405815 10.1152/ajpcell.1992.262.1.C199 10.1172/JCI118074 10.1073/pnas.92.14.6344 10.1006/jsre.1996.0358 10.1002/eji.1830240836 10.4049/jimmunol.145.1.283 10.1152/ajpcell.1996.270.6.C1815 10.1073/pnas.88.11.4651 10.1016/0306-3623(94)90120-1 10.1006/excr.1995.1275 10.1006/cimm.1996.0290 10.1159/000319584 10.1172/JCI116029 10.1073/pnas.90.13.6252 10.1182/blood.V86.9.3428.bloodjournal8693428 10.4049/jimmunol.159.2.614 |
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