Development of an Engineered Sugar Aminotransferase with Simultaneously Improved Stability and Non-Natural Substrate Activity to Synthesize the Glucosidase Inhibitor Valienamine

• Published in: Engineering (Beijing, China) Vol. 42; no. 11; pp. 185 - 195
• Main Authors: Wang, Runxi, Qiao, Lu, Liu, Mufei, Ran, Yanpeng, Wang, Jun, Yan, Wupeng, Feng, Yan, Cui, Li
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2024; State Key Laboratory of Microbial Metabolism & Joint International Research Laboratory of Metabolic and Developmental Sciences,School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China
• Subjects: Artificial reaction; Combinatorial active-site saturation test; Iterative saturation mutagenesis; Stability-activity trade-off; Sugar aminotransferase; Valienamine; Artificial reaction; Stability-activity trade-off; Valienamine; Sugar aminotransferase; Combinatorial active-site saturation test; Iterative saturation mutagenesis

[Display omitted] Sugar aminotransferases (SATs) catalyze the installation of chiral amines onto specific keto sugars, producing bioactive amino sugars. Their activity has been utilized in artificial reactions, such as using the SAT WecE to transform valienone into the valuable α-glucosidase inhibit...