Vascular leakage in the dorsal root ganglia of the rat, studied with horseradish peroxidase

Horseradish peroxidase (HRP) was found to pass from ganglionic blood vessels into the extracellular space of dorsal root and Vth nerve ganglia within 2 min of intravenous injection in the rat. By 5 min, the tracer had penetrated into perineuronal and periaxonal spaces. A brisk macrophage response oc...

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Bibliographic Details
Published inJournal of the neurological sciences Vol. 29; no. 1; pp. 95 - 107
Main Authors Jacobs, Jean M., Macfarlane, Rhona M., Cavanagh, J.B.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.09.1976
Subjects
Animals
Capillary Permeability
Cyproheptadine - pharmacology
Ganglia, Spinal - blood supply
Ganglia, Spinal - metabolism
Histocytochemistry
Horseradish Peroxidase
Macrophages - metabolism
Male
Mast Cells - metabolism
Rats
Spinal Nerves - metabolism
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Summary:Horseradish peroxidase (HRP) was found to pass from ganglionic blood vessels into the extracellular space of dorsal root and Vth nerve ganglia within 2 min of intravenous injection in the rat. By 5 min, the tracer had penetrated into perineuronal and periaxonal spaces. A brisk macrophage response occurred, and these cells rapidly engulfed the tracer so that it had almost completely disappeared from the extracellular space by 2–3 hr. Fenestrated blood vessels, with and without diaphragms were seen within ganglia. On the basis of the pattern of tracer distribution following post-fixation perfusion of HRP, it was concluded that the fenestrations and endothelial intercellular clefts were probably the most important route of tracer leakage. Because of the foreign and possibly toxic nature of the tracer, its movements and fate may not parallel that of normally extravasated proteins.
ISSN:0022-510X
1878-5883
DOI:10.1016/0022-510X(76)90083-6