Full-Length Transcriptome Profile of Apis cerana Revealed by Nanopore Sequencing
The Asian honey bee ( ) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three...
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Published in | International journal of molecular sciences Vol. 25; no. 19; p. 10833 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
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09.10.2024
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Abstract | The Asian honey bee (
) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of
and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identified 38,799 alternative splicing (AS) events from 5710 genes, 44,243 alternative polyadenylation (APA) sites from 1649 gene loci, 88,187 simple sequence repeats (SSRs), and 17,387 long noncoding RNAs (lncRNAs). Leveraging these transcripts as references, we identified 6672, 7795, and 6804 differentially expressed transcripts (DETs) in comparisons of queen ovaries vs drone testes, worker ovaries vs drone testes, and worker ovaries vs queen ovaries, respectively. Our research results provide a comprehensive set of reference transcript datasets for
, offering important sequence information for further exploration of its gene functions. |
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AbstractList | The Asian honey bee (Apis cerana) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of A. cerana and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identified 38,799 alternative splicing (AS) events from 5710 genes, 44,243 alternative polyadenylation (APA) sites from 1649 gene loci, 88,187 simple sequence repeats (SSRs), and 17,387 long noncoding RNAs (lncRNAs). Leveraging these transcripts as references, we identified 6672, 7795, and 6804 differentially expressed transcripts (DETs) in comparisons of queen ovaries vs drone testes, worker ovaries vs drone testes, and worker ovaries vs queen ovaries, respectively. Our research results provide a comprehensive set of reference transcript datasets for Apis cerana, offering important sequence information for further exploration of its gene functions. The Asian honey bee ( ) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identified 38,799 alternative splicing (AS) events from 5710 genes, 44,243 alternative polyadenylation (APA) sites from 1649 gene loci, 88,187 simple sequence repeats (SSRs), and 17,387 long noncoding RNAs (lncRNAs). Leveraging these transcripts as references, we identified 6672, 7795, and 6804 differentially expressed transcripts (DETs) in comparisons of queen ovaries vs drone testes, worker ovaries vs drone testes, and worker ovaries vs queen ovaries, respectively. Our research results provide a comprehensive set of reference transcript datasets for , offering important sequence information for further exploration of its gene functions. The Asian honey bee (Apis cerana) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of A. cerana and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identified 38,799 alternative splicing (AS) events from 5710 genes, 44,243 alternative polyadenylation (APA) sites from 1649 gene loci, 88,187 simple sequence repeats (SSRs), and 17,387 long noncoding RNAs (lncRNAs). Leveraging these transcripts as references, we identified 6672, 7795, and 6804 differentially expressed transcripts (DETs) in comparisons of queen ovaries vs drone testes, worker ovaries vs drone testes, and worker ovaries vs queen ovaries, respectively. Our research results provide a comprehensive set of reference transcript datasets for Apis cerana, offering important sequence information for further exploration of its gene functions.The Asian honey bee (Apis cerana) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of A. cerana and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identified 38,799 alternative splicing (AS) events from 5710 genes, 44,243 alternative polyadenylation (APA) sites from 1649 gene loci, 88,187 simple sequence repeats (SSRs), and 17,387 long noncoding RNAs (lncRNAs). Leveraging these transcripts as references, we identified 6672, 7795, and 6804 differentially expressed transcripts (DETs) in comparisons of queen ovaries vs drone testes, worker ovaries vs drone testes, and worker ovaries vs queen ovaries, respectively. Our research results provide a comprehensive set of reference transcript datasets for Apis cerana, offering important sequence information for further exploration of its gene functions. The Asian honey bee ( Apis cerana ) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of A. cerana and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology. Ultimately, we obtained 160,811 full-length transcript sequences from 19,859 genes, with 141,189 being novel transcripts, of which 130,367 were functionally annotated. We detected 520, 324, and 1823 specifically expressed transcripts in the queen, worker, and drone bees, respectively. Furthermore, we identified 38,799 alternative splicing (AS) events from 5710 genes, 44,243 alternative polyadenylation (APA) sites from 1649 gene loci, 88,187 simple sequence repeats (SSRs), and 17,387 long noncoding RNAs (lncRNAs). Leveraging these transcripts as references, we identified 6672, 7795, and 6804 differentially expressed transcripts (DETs) in comparisons of queen ovaries vs drone testes, worker ovaries vs drone testes, and worker ovaries vs queen ovaries, respectively. Our research results provide a comprehensive set of reference transcript datasets for Apis cerana , offering important sequence information for further exploration of its gene functions. |
Audience | Academic |
Author | Lin, Zong-Liang Hu, Xiao-Fen Jin, Meng-Jie Zeng, Zhi-Jiang Gong, Zhi-Xian Zhang, Li-Zhen Wang, Zi-Long |
AuthorAffiliation | 2 Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China 1 Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Nanchang 330045, China; hxfen999@aliyun.com (X.-F.H.); mengjiejin@icloud.com (M.-J.J.); ling15350309823@stu.jxau.edu.cn (Z.-X.G.); zlzcg@126.com (L.-Z.Z.); bees@jxau.edu.cn (Z.-J.Z.) 3 College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China |
AuthorAffiliation_xml | – name: 2 Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China – name: 1 Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Nanchang 330045, China; hxfen999@aliyun.com (X.-F.H.); mengjiejin@icloud.com (M.-J.J.); ling15350309823@stu.jxau.edu.cn (Z.-X.G.); zlzcg@126.com (L.-Z.Z.); bees@jxau.edu.cn (Z.-J.Z.) – name: 3 College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China |
Author_xml | – sequence: 1 givenname: Xiao-Fen surname: Hu fullname: Hu, Xiao-Fen organization: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China – sequence: 2 givenname: Meng-Jie surname: Jin fullname: Jin, Meng-Jie organization: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China – sequence: 3 givenname: Zhi-Xian surname: Gong fullname: Gong, Zhi-Xian organization: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China – sequence: 4 givenname: Zong-Liang surname: Lin fullname: Lin, Zong-Liang organization: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China – sequence: 5 givenname: Li-Zhen surname: Zhang fullname: Zhang, Li-Zhen organization: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China – sequence: 6 givenname: Zhi-Jiang orcidid: 0000-0001-5778-4115 surname: Zeng fullname: Zeng, Zhi-Jiang organization: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China – sequence: 7 givenname: Zi-Long surname: Wang fullname: Wang, Zi-Long organization: Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China |
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Cites_doi | 10.1261/rna.029751.111 10.1093/nar/gkt646 10.12688/f1000research.23297.1 10.1186/s12864-020-07014-x 10.1093/bioinformatics/bty191 10.1016/j.humimm.2021.02.012 10.1038/nprot.2013.084 10.1093/nar/gkm391 10.1038/nrg2776 10.3389/fgene.2020.00279 10.1038/s41598-021-90635-3 10.1186/1756-0500-3-123 10.1101/cshperspect.a036798 10.1186/1471-2164-16-1 10.1002/ps.6256 10.3390/ijms24065827 10.1101/gr.139733.112 10.1186/s12864-019-5838-3 10.1105/tpc.113.113803 10.1007/s00439-013-1411-3 10.1038/nrg.2015.10 10.1371/journal.pone.0047954 10.1007/s11427-013-4557-2 10.1016/j.celrep.2012.01.001 10.1371/journal.pgen.1006464 10.1038/ng.259 10.1038/ncomms11706 10.1093/bioinformatics/btr261 10.1101/gr.134106.111 10.1038/nrm.2016.116 10.1093/jee/toab186 10.1093/nar/gkt1223 10.1038/nature09616 10.1093/nar/gkm311 10.1371/journal.pone.0147147 10.1038/nature08909 10.1093/nar/gkt006 10.1371/journal.pntd.0008351 10.1016/j.gpb.2015.08.002 10.20944/preprints202301.0323.v1 10.1038/s41598-017-17338-6 10.1007/s00122-002-1031-0 |
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Keywords | differentially expressed transcripts nanopore sequencing alternative splicing full-length transcriptome Apis cerana |
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References | Li (ref_34) 2018; 34 ref_12 ref_33 ref_31 ref_30 Kong (ref_41) 2007; 35 Dong (ref_7) 2013; 56 Haas (ref_37) 2013; 8 Rhoads (ref_17) 2015; 13 Tian (ref_24) 2017; 18 ref_19 ref_18 Hamilton (ref_38) 2016; 7 Marquez (ref_11) 2012; 22 Smibert (ref_25) 2012; 1 Liu (ref_21) 2021; 114 Sun (ref_42) 2013; 41 Hu (ref_16) 2021; 82 Gamazon (ref_14) 2014; 133 Wang (ref_43) 2013; 41 Keren (ref_8) 2010; 11 Meng (ref_32) 2021; 77 Pertea (ref_36) 2020; 9 Ulitsky (ref_27) 2012; 22 ref_23 ref_22 ref_1 ref_3 McCombie (ref_15) 2019; 9 Jan (ref_26) 2011; 469 ref_2 ref_29 Shao (ref_20) 2012; 18 Nilsen (ref_9) 2010; 463 Foissac (ref_35) 2007; 35 Thiel (ref_40) 2003; 106 ref_5 Finn (ref_44) 2014; 42 ref_4 Quinn (ref_28) 2016; 17 Bailey (ref_39) 2011; 27 ref_6 Pan (ref_10) 2008; 40 Staiger (ref_13) 2013; 25 |
References_xml | – volume: 18 start-page: 1395 year: 2012 ident: ref_20 article-title: Alternative splicing and trans-splicing events revealed by analysis of the Bombyx mori transcriptome publication-title: RNA doi: 10.1261/rna.029751.111 contributor: fullname: Shao – volume: 41 start-page: e166 year: 2013 ident: ref_42 article-title: Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkt646 contributor: fullname: Sun – volume: 9 start-page: ISCB Comm J-304 year: 2020 ident: ref_36 article-title: GFF Utilities: GffRead and GffCompare publication-title: F1000Research doi: 10.12688/f1000research.23297.1 contributor: fullname: Pertea – ident: ref_30 doi: 10.1186/s12864-020-07014-x – volume: 34 start-page: 3094 year: 2018 ident: ref_34 article-title: Minimap2: Pairwise alignment for nucleotide sequences publication-title: Bioinformatics doi: 10.1093/bioinformatics/bty191 contributor: fullname: Li – volume: 82 start-page: 801 year: 2021 ident: ref_16 article-title: Next-generation sequencing technologies: An overview publication-title: Hum. Immunol. doi: 10.1016/j.humimm.2021.02.012 contributor: fullname: Hu – volume: 8 start-page: 1494 year: 2013 ident: ref_37 article-title: De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis publication-title: Nat. Protoc. doi: 10.1038/nprot.2013.084 contributor: fullname: Haas – volume: 35 start-page: W345 year: 2007 ident: ref_41 article-title: CPC: Assess the protein-coding potential of transcripts using sequence features and support vector machine publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkm391 contributor: fullname: Kong – volume: 11 start-page: 345 year: 2010 ident: ref_8 article-title: Alternative splicing and evolution: Diversification, exon definition and function publication-title: Nat. Rev. Genet. doi: 10.1038/nrg2776 contributor: fullname: Keren – ident: ref_4 doi: 10.3389/fgene.2020.00279 – ident: ref_6 doi: 10.1038/s41598-021-90635-3 – ident: ref_12 doi: 10.1186/1756-0500-3-123 – volume: 9 start-page: a036798 year: 2019 ident: ref_15 article-title: Next-Generation Sequencing Technologies publication-title: Cold Spring Harb. Perspect. Med. doi: 10.1101/cshperspect.a036798 contributor: fullname: McCombie – ident: ref_2 doi: 10.1186/1471-2164-16-1 – volume: 77 start-page: 2292 year: 2021 ident: ref_32 article-title: Genome-wide identification of long non-coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis publication-title: Pest Manag. Sci. doi: 10.1002/ps.6256 contributor: fullname: Meng – ident: ref_23 doi: 10.3390/ijms24065827 – volume: 22 start-page: 2054 year: 2012 ident: ref_27 article-title: Extensive alternative polyadenylation during zebrafish development publication-title: Genome Res. doi: 10.1101/gr.139733.112 contributor: fullname: Ulitsky – ident: ref_1 – ident: ref_22 doi: 10.1186/s12864-019-5838-3 – volume: 25 start-page: 3640 year: 2013 ident: ref_13 article-title: Alternative splicing at the intersection of biological timing, development, and stress responses publication-title: Plant Cell. doi: 10.1105/tpc.113.113803 contributor: fullname: Staiger – volume: 133 start-page: 679 year: 2014 ident: ref_14 article-title: Genomics of alternative splicing: Evolution, development and pathophysiology publication-title: Hum. Genet. doi: 10.1007/s00439-013-1411-3 contributor: fullname: Gamazon – volume: 17 start-page: 47 year: 2016 ident: ref_28 article-title: Unique features of long non-coding RNA biogenesis and function publication-title: Nat. Rev. Genet. doi: 10.1038/nrg.2015.10 contributor: fullname: Quinn – ident: ref_5 doi: 10.1371/journal.pone.0047954 – volume: 56 start-page: 960 year: 2013 ident: ref_7 article-title: Transcriptomics: Advances and approaches publication-title: Sci. China Life Sci. doi: 10.1007/s11427-013-4557-2 contributor: fullname: Dong – volume: 1 start-page: 277 year: 2012 ident: ref_25 article-title: Global patterns of tissue-specific alternative polyadenylation in Drosophila publication-title: Cell Rep. doi: 10.1016/j.celrep.2012.01.001 contributor: fullname: Smibert – ident: ref_19 doi: 10.1371/journal.pgen.1006464 – volume: 40 start-page: 1413 year: 2008 ident: ref_10 article-title: Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing publication-title: Nat. Genet. doi: 10.1038/ng.259 contributor: fullname: Pan – ident: ref_33 – volume: 7 start-page: 11706 year: 2016 ident: ref_38 article-title: A survey of the sorghum transcriptome using single-molecule long reads publication-title: Nat. Commun. doi: 10.1038/ncomms11706 contributor: fullname: Hamilton – volume: 27 start-page: 1653 year: 2011 ident: ref_39 article-title: DREME: Motif discovery in transcription factor ChIP-seq data publication-title: Bioinformatics doi: 10.1093/bioinformatics/btr261 contributor: fullname: Bailey – volume: 22 start-page: 1184 year: 2012 ident: ref_11 article-title: Transcriptome survey reveals increased complexity of the alternative splicing landscape in Arabidopsis publication-title: Genome Res. doi: 10.1101/gr.134106.111 contributor: fullname: Marquez – volume: 18 start-page: 18 year: 2017 ident: ref_24 article-title: Alternative polyadenylation of mRNA precursors publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/nrm.2016.116 contributor: fullname: Tian – volume: 114 start-page: 2512 year: 2021 ident: ref_21 article-title: Genome-wide Analysis of Alternative Gene Splicing Associated with Virulence in the Brown Planthopper Nilaparvata lugens (Hemiptera: Delphacidae) publication-title: J. Econ. Entomol. doi: 10.1093/jee/toab186 contributor: fullname: Liu – volume: 42 start-page: D222 year: 2014 ident: ref_44 article-title: Pfam: The protein families database publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkt1223 contributor: fullname: Finn – volume: 469 start-page: 97 year: 2011 ident: ref_26 article-title: Formation, regulation and evolution of Caenorhabditis elegans 3’UTRs publication-title: Nature doi: 10.1038/nature09616 contributor: fullname: Jan – volume: 35 start-page: W297 year: 2007 ident: ref_35 article-title: ASTALAVISTA: Dynamic and flexible analysis of alternative splicing events in custom gene datasets publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkm311 contributor: fullname: Foissac – ident: ref_29 doi: 10.1371/journal.pone.0147147 – volume: 463 start-page: 457 year: 2010 ident: ref_9 article-title: Expansion of the eukaryotic proteome by alternative splicing publication-title: Nature doi: 10.1038/nature08909 contributor: fullname: Nilsen – volume: 41 start-page: e74 year: 2013 ident: ref_43 article-title: CPAT: Coding-Potential Assessment Tool using an alignment-free logistic regression model publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkt006 contributor: fullname: Wang – ident: ref_31 doi: 10.1371/journal.pntd.0008351 – volume: 13 start-page: 278 year: 2015 ident: ref_17 article-title: PacBio Sequencing and Its Applications publication-title: Genom. Proteom. Bioinf. doi: 10.1016/j.gpb.2015.08.002 contributor: fullname: Rhoads – ident: ref_18 doi: 10.20944/preprints202301.0323.v1 – ident: ref_3 doi: 10.1038/s41598-017-17338-6 – volume: 106 start-page: 411 year: 2003 ident: ref_40 article-title: Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) publication-title: Theor. Appl. Genet. doi: 10.1007/s00122-002-1031-0 contributor: fullname: Thiel |
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Snippet | The Asian honey bee (
) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic... The Asian honey bee (Apis cerana) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of... The Asian honey bee ( Apis cerana ) plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of... |
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SubjectTerms | Alternative Splicing Animals Apis cerana Bees Bees - genetics differentially expressed transcripts DNA sequencing Female full-length transcriptome Gene Expression Profiling - methods Genes Genomes Genomics Honey Honeybee Male Microsatellite Repeats - genetics Molecular Sequence Annotation nanopore sequencing Nanopore Sequencing - methods Nucleotide sequencing Ovaries Polyadenylation - genetics Polymers Proteins RNA, Long Noncoding - genetics Sequence Analysis, RNA - methods Technology Testes Transcriptome - genetics |
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Title | Full-Length Transcriptome Profile of Apis cerana Revealed by Nanopore Sequencing |
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