Localization of Phosphatidylserine Binding Sites to Structural Domains of Factor Xa

• Published in: The Journal of biological chemistry Vol. 277; no. 3; pp. 1855 - 1863
• Main Authors: Srivastava, Arvind, Wang, Jianfang, Majumder, Rinku, Rezaie, Alireza R., Stenflo, Johan, Esmon, Charles T., Lentz, Barry R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.01.2002; American Society for Biochemistry and Molecular Biology
• Subjects: Amides - metabolism; Animals; Binding Sites; Cattle; Circular Dichroism; Factor Xa - chemistry; Factor Xa - metabolism; Humans; Hydrolysis; Phosphatidylserines - metabolism; Protein Conformation; Spectrometry, Fluorescence

Binding of short chain phosphatidylserine (C6PS) enhances the proteolytic activity of factor Xa by 60-fold (Koppaka, V., Wang, J., Banerjee, M., and Lentz, B. R. (1996) Biochemistry 35, 7482–7491). In the present study, we locate three C6PS binding sites to different domains of factor Xa using a com...