Influenza virus subpopulations: exchange of lethal H5N1 virus NS for H1N1 virus NS triggers de novo generation of defective-interfering particles and enhances interferon-inducing particle efficiency

• Published in: Journal of interferon & cytokine research Vol. 33; no. 3; pp. 99 - 107
• Main Authors: Ngunjiri, John M, Buchek, Gregory M, Mohni, Kareem N, Sekellick, Margaret J, Marcus, Philip I
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.03.2013
• Subjects: Amino Acid Sequence; Animals; Cells, Cultured; Chick Embryo; Humans; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H5N1 Subtype - genetics; Influenza A Virus, H5N1 Subtype - immunology; Influenza Vaccines - genetics; Influenza Vaccines - immunology; Interferons - immunology; Sequence Deletion; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - immunology

Reassortment of influenza A viruses is known to affect viability, replication efficiency, antigenicity, host range, and virulence, and can generate pandemic strains. In this study, we demonstrated that the specific exchange of the NS gene segment from highly pathogenic A/HK/156/97 (H5N1) [E92 or E92...