Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes

• Published in: Chromosoma Vol. 60; no. 1; p. 15
• Main Authors: Lin, M S, Comings, D E, Alfi, O S
• Format: Journal Article
• Language: English
• Published: Austria 07.03.1977
• Subjects: Adenine Nucleotides - analysis; Amidines; Animals; Binding Sites; Bromodeoxyuridine; Chromosomes - analysis; DNA - analysis; Fluorescent Dyes; Humans; Hydrogen-Ion Concentration; Indoles; Mice; Mitosis; Polynucleotides - analysis; Spectrometry, Fluorescence; Staining and Labeling; Thymine Nucleotides - analysis

The optical absorption and fluorescence characteristics of 4'-6-diamidino-2-phenylindole (DAPI) with DNA and chromosomes were studied. There is a decrease in extinction coefficient and chift in the absorption spectra to a higher wavelength when the dye binds to DNA. The fluorescence of DAPI is...