A dual-fluorescent reporter facilitates identification of thiol compounds that suppress microsatellite instability induced by oxidative stress
The DNA mismatch-repair (MMR) system corrects replicative errors and minimizes mutations that occur at a high rate in microsatellites. Patients with chronic inflammation or inflammation-associated cancer display microsatellite instability (MSI), indicating a possible MMR inactivation. In fact, H2O2-...
Saved in:
Published in | Free radical biology & medicine Vol. 69; pp. 86 - 95 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.04.2014
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The DNA mismatch-repair (MMR) system corrects replicative errors and minimizes mutations that occur at a high rate in microsatellites. Patients with chronic inflammation or inflammation-associated cancer display microsatellite instability (MSI), indicating a possible MMR inactivation. In fact, H2O2-generated oxidative stress inactivates the MMR function and increases mutation accumulation in a reporter microsatellite. However, it remains unclear whether MSI induced by oxidative stress is preventable because of the lack of a sufficiently sensitive detection assay. Here, we developed and characterized a dual-fluorescent system, utilizing DsRed harboring the (CA)13 microsatellite as a reporter and GFP for normalization, in near-isogenic human colorectal cancer cell lines. Via flow cytometry, this reporter sensitively detected H2O2-generated oxidative microsatellite mutations in a dose-dependent manner. The reporter further revealed that glutathione or N-acetylcysteine was better than aspirin and ascorbic acid for suppressing oxidative microsatellite mutations. These two thiol compounds also partially suppressed oxidative frameshift mutations in the coding microsatellites of the hMSH6 and CHK1 genes based on a fluoresceinated PCR-based assay. MSI suppression by N-acetylcysteine appears to be mediated through reduction of oxidative frameshift mutations in the coding microsatellite of hMSH6 and protection of hMSH6 and other MMR protein levels from being decreased by H2O2. Our findings suggest a linkage between oxidative damage, MMR deficiency, and MSI. The two thiol compounds are potentially valuable for preventing inflammation-associated MSI. The dual-fluorescent reporter with improved features will facilitate identification of additional compounds that modulate MSI, which is relevant to cancer initiation and progression. |
---|---|
AbstractList | The DNA mismatch-repair (MMR) system corrects replicative errors and minimizes mutations that occur at a high rate in microsatellites. Patients with chronic inflammation or inflammation-associated cancer display microsatellite instability (MSI), indicating a possible MMR inactivation. In fact, H2O2-generated oxidative stress inactivates the MMR function and increases mutation accumulation in a reporter microsatellite. However, it remains unclear whether MSI induced by oxidative stress is preventable because of the lack of a sufficiently sensitive detection assay. Here, we developed and characterized a dual-fluorescent system, utilizing DsRed harboring the (CA)13 microsatellite as a reporter and GFP for normalization, in near-isogenic human colorectal cancer cell lines. Via flow cytometry, this reporter sensitively detected H2O2-generated oxidative microsatellite mutations in a dose-dependent manner. The reporter further revealed that glutathione or N-acetylcysteine was better than aspirin and ascorbic acid for suppressing oxidative microsatellite mutations. These two thiol compounds also partially suppressed oxidative frameshift mutations in the coding microsatellites of the hMSH6 and CHK1 genes based on a fluoresceinated PCR-based assay. MSI suppression by N-acetylcysteine appears to be mediated through reduction of oxidative frameshift mutations in the coding microsatellite of hMSH6 and protection of hMSH6 and other MMR protein levels from being decreased by H2O2. Our findings suggest a linkage between oxidative damage, MMR deficiency, and MSI. The two thiol compounds are potentially valuable for preventing inflammation-associated MSI. The dual-fluorescent reporter with improved features will facilitate identification of additional compounds that modulate MSI, which is relevant to cancer initiation and progression. |
Author | Chang, Christina L Li, I-Chen Chiu, Chien-Yuan Jian, Siao-Ru Wang, Shainn-Wei Wu, Chang-Lin Chi, Jhih-Ying |
Author_xml | – sequence: 1 givenname: I-Chen surname: Li fullname: Li, I-Chen organization: Institute of Molecular Medicine, National Cheng Kung University, Tainan 70101, Taiwan – sequence: 2 givenname: Chien-Yuan surname: Chiu fullname: Chiu, Chien-Yuan organization: Institute of Oral Medicine, and National Cheng Kung University, Tainan 70101, Taiwan – sequence: 3 givenname: Chang-Lin surname: Wu fullname: Wu, Chang-Lin organization: Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 70101, Taiwan – sequence: 4 givenname: Jhih-Ying surname: Chi fullname: Chi, Jhih-Ying organization: Institute of Molecular Medicine, National Cheng Kung University, Tainan 70101, Taiwan – sequence: 5 givenname: Siao-Ru surname: Jian fullname: Jian, Siao-Ru organization: Institute of Oral Medicine, and National Cheng Kung University, Tainan 70101, Taiwan – sequence: 6 givenname: Shainn-Wei surname: Wang fullname: Wang, Shainn-Wei organization: Institute of Molecular Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 70101, Taiwan – sequence: 7 givenname: Christina L surname: Chang fullname: Chang, Christina L email: clchang@mail.ncku.edu.tw organization: Institute of Molecular Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Institute of Oral Medicine, and National Cheng Kung University, Tainan 70101, Taiwan; Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 70101, Taiwan. Electronic address: clchang@mail.ncku.edu.tw |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24412704$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkc1q3TAQhUVJaG5-XqEIuunGjmTJskxXIaRtINBNuxb6GVFdbMuV5NL7EnnmyiRZdDUazpxPHM4lOlviAgh9pKSlhIrbY-sTQNLOhDiDaztCWUu7ltDxHTpQObCG96M4QwciR9r0ko8X6DLnIyGE90y-Rxcd57QbCD-g5zvsNj01ftpigmxhKTjBGlOBhL22YQpFF8g4uCoFH6wuIS44elx-hThhG-c1bovLddcF521dKyfjOdgUc7VOlQA4LLlos9NO9e02Cw6bE45_g6vAP4Bz2W3X6NzrKcPN67xCP788_Lj_1jx9__p4f_fUWNbR0nhWk3jOmTUWOjY44ahgvRkG76oy6GEkxDlrjTSc0hGE7BmnQhAmescMu0KfXrhrir83yEXNoYafJr1A3LKiPZFCUinGevr55XTPkxN4taYw63RSlKi9EHVU_xWi9kIU7VQtpLo_vH60mV178741wP4BTdaSNg |
CitedBy_id | crossref_primary_10_1016_j_freeradbiomed_2017_07_006 crossref_primary_10_1186_s12906_015_0765_6 |
Cites_doi | 10.1093/jjco/hyh018 10.3748/wjg.14.6717 10.1053/gast.1997.v113.pm9322509 10.1007/s00384-008-0606-x 10.1096/fasebj.13.9.1007 10.1073/pnas.0511304103 10.1089/152308601750100687 10.1073/pnas.95.12.6870 10.1152/ajpcell.00283.2008 10.1089/ars.2013.5529 10.1093/carcin/bgp127 10.1126/science.1114233 10.1016/S0960-9822(02)00863-1 10.4049/jimmunol.170.4.2214 10.1586/14737140.1.1.149 10.1007/s10350-008-9212-9 10.1016/j.molcel.2009.06.030 10.1016/S0027-5107(98)00227-9 10.1093/carcin/18.6.1127 10.1016/j.molcel.2010.09.019 10.1155/2004/136734 10.1093/nar/gkp839 10.1038/nrg1348 10.1073/pnas.95.19.11301 10.1016/j.coph.2007.04.005 10.1073/pnas.95.21.12468 10.1172/JCI19757 10.1007/s000180300001 10.1111/j.1440-1746.2005.03803.x 10.1093/carcin/bgm101 10.1016/j.annemergmed.2004.09.028 10.1038/nrclinonc.2010.18 10.1016/S1568-7864(02)00113-1 10.1093/nar/gki813 10.1016/S1097-2765(00)80346-9 10.1093/nar/12.4.2137 10.1038/349431a0 10.1007/s00726-010-0610-7 10.1158/0008-5472.CAN-03-2992 10.1158/1541-7786.MCR-09-0299 10.1002/emmm.200900040 10.1093/jnci/djh034 10.1111/j.1572-0241.1998.00592.x 10.1093/carcin/22.10.1709 10.1002/(SICI)1097-0142(19980515)82:10<1808::AID-CNCR2>3.0.CO;2-J 10.1128/MCB.24.1.465-474.2004 10.1152/ajpcell.00422.2001 10.1074/jbc.M111269200 10.1007/s10620-011-1825-5 10.1158/0008-5472.CAN-04-3824 10.1007/s00280-010-1418-6 10.1200/JCO.2003.04.060 10.1146/annurev.biochem.74.082803.133243 10.1038/onc.2009.421 10.1172/JCI200320502 10.1158/1078-0432.CCR-08-2686 10.1074/jbc.M108618200 |
ContentType | Journal Article |
Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Copyright_xml | – notice: Copyright © 2013 Elsevier Inc. All rights reserved. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1016/j.freeradbiomed.2013.12.019 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology Biology |
EISSN | 1873-4596 |
EndPage | 95 |
ExternalDocumentID | 10_1016_j_freeradbiomed_2013_12_019 24412704 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M -~X .GJ .HR .~1 0R~ 1B1 1RT 1~. 1~5 29H 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM AABNK AACTN AAEDT AAEDW AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXKI AAXUO ABBQC ABFNM ABFRF ABGSF ABJNI ABLJU ABMAC ABMZM ABUDA ABXDB ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFJKZ AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJOXV AJRQY AKRWK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV C45 CGR CS3 CUY CVF DU5 EBS ECM EFJIC EIF EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HEA HLW HMK HMO HVGLF HX~ HZ~ IHE J1W KOM LX3 LZ2 M29 M41 MO0 N9A NPM O-L O9- OAUVE OVD OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SAE SBG SCC SDF SDG SDP SES SEW SPCBC SSH SSU SSZ T5K TEORI WUQ XPP ZGI ~G- AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c321t-f3045f443cbce237d6d1635b77fd0457a7900ddccb8b4119e685341660365d3b3 |
ISSN | 0891-5849 |
IngestDate | Sat Oct 05 04:34:30 EDT 2024 Thu Sep 26 15:56:37 EDT 2024 Sat Sep 28 07:57:33 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | Oxidative stress Free radicals Colorectal cancer Dual-fluorescent reporter Inflammation Microsatellite instability DNA mismatch repair Glutathione N-acetylcysteine |
Language | English |
License | Copyright © 2013 Elsevier Inc. All rights reserved. |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c321t-f3045f443cbce237d6d1635b77fd0457a7900ddccb8b4119e685341660365d3b3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 24412704 |
PQID | 1508681869 |
PQPubID | 23479 |
PageCount | 10 |
ParticipantIDs | proquest_miscellaneous_1508681869 crossref_primary_10_1016_j_freeradbiomed_2013_12_019 pubmed_primary_24412704 |
PublicationCentury | 2000 |
PublicationDate | 2014-Apr 2014-04-00 20140401 |
PublicationDateYYYYMMDD | 2014-04-01 |
PublicationDate_xml | – month: 04 year: 2014 text: 2014-Apr |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Free radical biology & medicine |
PublicationTitleAlternate | Free Radic Biol Med |
PublicationYear | 2014 |
References | Hewish (10.1016/j.freeradbiomed.2013.12.019_bib12) 2010; 7 Colotta (10.1016/j.freeradbiomed.2013.12.019_bib15) 2009; 30 Russo (10.1016/j.freeradbiomed.2013.12.019_bib49) 2004; 24 Ni (10.1016/j.freeradbiomed.2013.12.019_bib2) 1999; 4 Tirouvanziam (10.1016/j.freeradbiomed.2013.12.019_bib72) 2006; 103 Mirzaee (10.1016/j.freeradbiomed.2013.12.019_bib24) 2008; 14 Lee (10.1016/j.freeradbiomed.2013.12.019_bib29) 2012; 57 Brentnall (10.1016/j.freeradbiomed.2013.12.019_bib17) 1996; 56 Ohzeki (10.1016/j.freeradbiomed.2013.12.019_bib48) 1997; 18 Ruschoff (10.1016/j.freeradbiomed.2013.12.019_bib37) 1998; 95 O'Donovan (10.1016/j.freeradbiomed.2013.12.019_bib67) 2005; 309 Park (10.1016/j.freeradbiomed.2013.12.019_bib19) 1998; 2 Colussi (10.1016/j.freeradbiomed.2013.12.019_bib54) 2002; 12 Chang (10.1016/j.freeradbiomed.2013.12.019_bib10) 2002; 283 Zafarullah (10.1016/j.freeradbiomed.2013.12.019_bib69) 2003; 60 Harreus (10.1016/j.freeradbiomed.2013.12.019_bib65) 2005; 25 Cravo (10.1016/j.freeradbiomed.2013.12.019_bib18) 1998; 93 Herman (10.1016/j.freeradbiomed.2013.12.019_bib9) 1998; 95 Martin (10.1016/j.freeradbiomed.2013.12.019_bib36) 2009; 1 Kasai (10.1016/j.freeradbiomed.2013.12.019_bib42) 2007; 12 Egashira (10.1016/j.freeradbiomed.2013.12.019_bib47) 2002; 1 Fleisher (10.1016/j.freeradbiomed.2013.12.019_bib20) 2000; 60 Prescott (10.1016/j.freeradbiomed.2013.12.019_bib71) 2005; 45 Arzimanoglou (10.1016/j.freeradbiomed.2013.12.019_bib5) 1998; 82 Shibutani (10.1016/j.freeradbiomed.2013.12.019_bib43) 1991; 349 Risinger (10.1016/j.freeradbiomed.2013.12.019_bib7) 1993; 53 Klaunig (10.1016/j.freeradbiomed.2013.12.019_bib16) 1998; 106 Peltomaki (10.1016/j.freeradbiomed.2013.12.019_bib4) 1997; 113 Takemoto (10.1016/j.freeradbiomed.2013.12.019_bib30) 2004; 34 Aaltonen (10.1016/j.freeradbiomed.2013.12.019_bib6) 1994; 54 Bacher (10.1016/j.freeradbiomed.2013.12.019_bib57) 2004; 20 Ciccia (10.1016/j.freeradbiomed.2013.12.019_bib59) 2010; 40 Gasche (10.1016/j.freeradbiomed.2013.12.019_bib61) 2005; 65 Naganuma (10.1016/j.freeradbiomed.2013.12.019_bib25) 2004; 64 Carr (10.1016/j.freeradbiomed.2013.12.019_bib63) 1999; 13 Fujiwara (10.1016/j.freeradbiomed.2013.12.019_bib28) 2008; 51 Wang (10.1016/j.freeradbiomed.2013.12.019_bib38) 2010; 29 Mazurek (10.1016/j.freeradbiomed.2013.12.019_bib45) 2002; 277 Liu (10.1016/j.freeradbiomed.2013.12.019_bib53) 2002; 8 10.1016/j.freeradbiomed.2013.12.019_bib44 Gebicki (10.1016/j.freeradbiomed.2013.12.019_bib70) 2010; 39 Sagher (10.1016/j.freeradbiomed.2013.12.019_bib11) 1999; 423 Gu (10.1016/j.freeradbiomed.2013.12.019_bib46) 2002; 277 Irving (10.1016/j.freeradbiomed.2013.12.019_bib14) 2001; 1 Koi (10.1016/j.freeradbiomed.2013.12.019_bib32) 1994; 54 Warusavitarne (10.1016/j.freeradbiomed.2013.12.019_bib60) 2009; 24 Umar (10.1016/j.freeradbiomed.2013.12.019_bib41) 2004; 96 Hofseth (10.1016/j.freeradbiomed.2013.12.019_bib55) 2003; 112 Suzuki (10.1016/j.freeradbiomed.2013.12.019_bib26) 1994; 54 Bonanomi (10.1016/j.freeradbiomed.2013.12.019_bib68) 1980; 111 Guo (10.1016/j.freeradbiomed.2013.12.019_bib56) 2003; 112 Boland (10.1016/j.freeradbiomed.2013.12.019_bib33) 1998; 58 Machado (10.1016/j.freeradbiomed.2013.12.019_bib23) 2009; 15 Gasche (10.1016/j.freeradbiomed.2013.12.019_bib31) 2001; 61 Mazurek (10.1016/j.freeradbiomed.2013.12.019_bib3) 2002; 277 Luciani (10.1016/j.freeradbiomed.2013.12.019_bib62) 2013; 28 Fromberg (10.1016/j.freeradbiomed.2013.12.019_bib66) 2011; 67 Brentnall (10.1016/j.freeradbiomed.2013.12.019_bib22) 1995; 55 Jackson (10.1016/j.freeradbiomed.2013.12.019_bib51) 1998; 95 Glaab (10.1016/j.freeradbiomed.2013.12.019_bib39) 2001; 22 Zhang (10.1016/j.freeradbiomed.2013.12.019_bib58) 2009; 35 Macpherson (10.1016/j.freeradbiomed.2013.12.019_bib50) 2005; 33 Lee (10.1016/j.freeradbiomed.2013.12.019_bib21) 2003; 170 Clement (10.1016/j.freeradbiomed.2013.12.019_bib64) 2001; 3 Woerner (10.1016/j.freeradbiomed.2013.12.019_bib13) 2010; 38 Jones (10.1016/j.freeradbiomed.2013.12.019_bib35) 2008; 295 Kunkel (10.1016/j.freeradbiomed.2013.12.019_bib1) 1996; 74 Tahara (10.1016/j.freeradbiomed.2013.12.019_bib27) 2005; 20 Peltomaki (10.1016/j.freeradbiomed.2013.12.019_bib8) 2003; 21 Atkuri (10.1016/j.freeradbiomed.2013.12.019_bib40) 2007; 7 Liu (10.1016/j.freeradbiomed.2013.12.019_bib52) 2009; 7 Ellegren (10.1016/j.freeradbiomed.2013.12.019_bib34) 2004; 5 |
References_xml | – volume: 34 start-page: 90 year: 2004 ident: 10.1016/j.freeradbiomed.2013.12.019_bib30 article-title: The correlation of microsatellite instability and tumor-infiltrating lymphocytes in hereditary non-polyposis colorectal cancer (HNPCC) and sporadic colorectal cancers: the significance of different types of lymphocyte infiltration publication-title: Jpn. J. Clin. Oncol. doi: 10.1093/jjco/hyh018 contributor: fullname: Takemoto – volume: 14 start-page: 6717 year: 2008 ident: 10.1016/j.freeradbiomed.2013.12.019_bib24 article-title: Helicobacter pylori infection and expression of DNA mismatch repair proteins publication-title: World J. Gastroenterol. doi: 10.3748/wjg.14.6717 contributor: fullname: Mirzaee – volume: 113 start-page: 1146 year: 1997 ident: 10.1016/j.freeradbiomed.2013.12.019_bib4 article-title: Mutations predisposing to hereditary nonpolyposis colorectal cancer: database and results of a collaborative study. The International Collaborative Group on Hereditary Nonpolyposis Colorectal Cancer publication-title: Gastroenterology doi: 10.1053/gast.1997.v113.pm9322509 contributor: fullname: Peltomaki – volume: 24 start-page: 139 year: 2009 ident: 10.1016/j.freeradbiomed.2013.12.019_bib60 article-title: Restoring TGFbeta function in microsatellite unstable (MSI-H) colorectal cancer reduces tumourigenicity but increases metastasis formation publication-title: Int. J. Colorectal Dis. doi: 10.1007/s00384-008-0606-x contributor: fullname: Warusavitarne – volume: 13 start-page: 1007 year: 1999 ident: 10.1016/j.freeradbiomed.2013.12.019_bib63 article-title: Does vitamin C act as a pro-oxidant under physiological conditions? publication-title: FASEB J doi: 10.1096/fasebj.13.9.1007 contributor: fullname: Carr – volume: 55 start-page: 4264 year: 1995 ident: 10.1016/j.freeradbiomed.2013.12.019_bib22 article-title: Microsatellite instability and K-ras mutations associated with pancreatic adenocarcinoma and pancreatitis publication-title: Cancer Res. contributor: fullname: Brentnall – volume: 103 start-page: 4628 year: 2006 ident: 10.1016/j.freeradbiomed.2013.12.019_bib72 article-title: High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.0511304103 contributor: fullname: Tirouvanziam – volume: 3 start-page: 157 year: 2001 ident: 10.1016/j.freeradbiomed.2013.12.019_bib64 article-title: The in vitro cytotoxicity of ascorbate depends on the culture medium used to perform the assay and involves hydrogen peroxide publication-title: Antioxid. Redox Signaling doi: 10.1089/152308601750100687 contributor: fullname: Clement – volume: 95 start-page: 6870 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib9 article-title: Incidence and functional consequences of hMLH1 promoter hypermethylation in colorectal carcinoma publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.95.12.6870 contributor: fullname: Herman – volume: 106 start-page: 289 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib16 article-title: The role of oxidative stress in chemical carcinogenesis publication-title: Environ. Health Perspect. contributor: fullname: Klaunig – volume: 295 start-page: C849 year: 2008 ident: 10.1016/j.freeradbiomed.2013.12.019_bib35 article-title: Radical-free biology of oxidative stress publication-title: Am. J. Physiol. Cell Physiol doi: 10.1152/ajpcell.00283.2008 contributor: fullname: Jones – ident: 10.1016/j.freeradbiomed.2013.12.019_bib44 doi: 10.1089/ars.2013.5529 – volume: 111 start-page: 45 year: 1980 ident: 10.1016/j.freeradbiomed.2013.12.019_bib68 article-title: Toxicological, pharmacokinetic and metabolic studies on acetylcysteine publication-title: Eur. J. Respir. Dis contributor: fullname: Bonanomi – volume: 30 start-page: 1073 year: 2009 ident: 10.1016/j.freeradbiomed.2013.12.019_bib15 article-title: Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability publication-title: Carcinogenesis doi: 10.1093/carcin/bgp127 contributor: fullname: Colotta – volume: 309 start-page: 1871 year: 2005 ident: 10.1016/j.freeradbiomed.2013.12.019_bib67 article-title: light generate mutagenic oxidative DNA damage publication-title: Science doi: 10.1126/science.1114233 contributor: fullname: O'Donovan – volume: 12 start-page: 912 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib54 article-title: The mammalian mismatch repair pathway removes DNA 8-oxodGMP incorporated from the oxidized dNTP pool publication-title: Curr. Biol. doi: 10.1016/S0960-9822(02)00863-1 contributor: fullname: Colussi – volume: 170 start-page: 2214 year: 2003 ident: 10.1016/j.freeradbiomed.2013.12.019_bib21 article-title: Microsatellite instability and suppressed DNA repair enzyme expression in rheumatoid arthritis publication-title: J. Immunol. doi: 10.4049/jimmunol.170.4.2214 contributor: fullname: Lee – volume: 1 start-page: 149 year: 2001 ident: 10.1016/j.freeradbiomed.2013.12.019_bib14 article-title: Mismatch repair defects as a cause of resistance to cytotoxic drugs publication-title: Expert Rev. Anticancer Ther. doi: 10.1586/14737140.1.1.149 contributor: fullname: Irving – volume: 60 start-page: 4864 year: 2000 ident: 10.1016/j.freeradbiomed.2013.12.019_bib20 article-title: Microsatellite instability in inflammatory bowel disease-associated neoplastic lesions is associated with hypermethylation and diminished expression of the DNA mismatch repair gene, hMLH1 publication-title: Cancer Res. contributor: fullname: Fleisher – volume: 54 start-page: 4841 year: 1994 ident: 10.1016/j.freeradbiomed.2013.12.019_bib26 article-title: Microsatellite instability in ulcerative colitis-associated colorectal dysplasias and cancers publication-title: Cancer Res. contributor: fullname: Suzuki – volume: 51 start-page: 1387 year: 2008 ident: 10.1016/j.freeradbiomed.2013.12.019_bib28 article-title: Ulcerative colitis-associated colorectal cancer is frequently associated with the microsatellite instability pathway publication-title: Dis. Colon Rectum doi: 10.1007/s10350-008-9212-9 contributor: fullname: Fujiwara – volume: 35 start-page: 442 year: 2009 ident: 10.1016/j.freeradbiomed.2013.12.019_bib58 article-title: The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress publication-title: Mol. Cell doi: 10.1016/j.molcel.2009.06.030 contributor: fullname: Zhang – volume: 423 start-page: 73 year: 1999 ident: 10.1016/j.freeradbiomed.2013.12.019_bib11 article-title: Stabilization of the intermediate in frameshift mutation publication-title: Mutat. Res. doi: 10.1016/S0027-5107(98)00227-9 contributor: fullname: Sagher – volume: 58 start-page: 5248 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib33 article-title: A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer publication-title: Cancer Res. contributor: fullname: Boland – volume: 18 start-page: 1127 year: 1997 ident: 10.1016/j.freeradbiomed.2013.12.019_bib48 article-title: Spectra of spontaneous mutations at the hprt locus in colorectal carcinoma cell lines defective in mismatch repair publication-title: Carcinogenesis doi: 10.1093/carcin/18.6.1127 contributor: fullname: Ohzeki – volume: 40 start-page: 179 year: 2010 ident: 10.1016/j.freeradbiomed.2013.12.019_bib59 article-title: The DNA damage response: making it safe to play with knives publication-title: Mol. Cell doi: 10.1016/j.molcel.2010.09.019 contributor: fullname: Ciccia – volume: 20 start-page: 237 year: 2004 ident: 10.1016/j.freeradbiomed.2013.12.019_bib57 article-title: Development of a fluorescent multiplex assay for detection of MSI-High tumors publication-title: Dis. Markers doi: 10.1155/2004/136734 contributor: fullname: Bacher – volume: 53 start-page: 5100 year: 1993 ident: 10.1016/j.freeradbiomed.2013.12.019_bib7 article-title: Genetic instability of microsatellites in endometrial carcinoma publication-title: Cancer Res. contributor: fullname: Risinger – volume: 38 start-page: D682 year: 2010 ident: 10.1016/j.freeradbiomed.2013.12.019_bib13 article-title: SelTarbase, a database of human mononucleotide-microsatellite mutations and their potential impact to tumorigenesis and immunology publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkp839 contributor: fullname: Woerner – volume: 5 start-page: 435 year: 2004 ident: 10.1016/j.freeradbiomed.2013.12.019_bib34 article-title: Microsatellites: simple sequences with complex evolution publication-title: Nat. Rev. Genet. doi: 10.1038/nrg1348 contributor: fullname: Ellegren – volume: 95 start-page: 11301 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib37 article-title: Aspirin suppresses the mutator phenotype associated with hereditary nonpolyposis colorectal cancer by genetic selection publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.95.19.11301 contributor: fullname: Ruschoff – volume: 7 start-page: 355 year: 2007 ident: 10.1016/j.freeradbiomed.2013.12.019_bib40 article-title: N-acetylcysteine—a safe antidote for cysteine/glutathione deficiency publication-title: Curr. Opin. Pharmacol. doi: 10.1016/j.coph.2007.04.005 contributor: fullname: Atkuri – volume: 95 start-page: 12468 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib51 article-title: Induction of microsatellite instability by oxidative DNA damage publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.95.21.12468 contributor: fullname: Jackson – volume: 112 start-page: 1887 year: 2003 ident: 10.1016/j.freeradbiomed.2013.12.019_bib55 article-title: The adaptive imbalance in base excision-repair enzymes generates microsatellite instability in chronic inflammation publication-title: J. Clin. Invest. doi: 10.1172/JCI19757 contributor: fullname: Hofseth – volume: 2 start-page: 221 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib19 article-title: Loss of heterozygosity and microsatellite instability in non-neoplastic mucosa from patients with chronic ulcerative colitis publication-title: Int. J. Mol. Med. contributor: fullname: Park – volume: 8 start-page: 2985 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib53 article-title: Base excision repair as a therapeutic target in colon cancer publication-title: Clin. Cancer Res. contributor: fullname: Liu – volume: 60 start-page: 6 year: 2003 ident: 10.1016/j.freeradbiomed.2013.12.019_bib69 article-title: Molecular mechanisms of N-acetylcysteine actions publication-title: Cell Mol. Life Sci. doi: 10.1007/s000180300001 contributor: fullname: Zafarullah – volume: 20 start-page: 710 year: 2005 ident: 10.1016/j.freeradbiomed.2013.12.019_bib27 article-title: Clinical significance of microsatellite instability in the inflamed mucosa for the prediction of colonic neoplasms in patients with ulcerative colitis publication-title: J. Gastroenterol. Hepatol. doi: 10.1111/j.1440-1746.2005.03803.x contributor: fullname: Tahara – volume: 28 start-page: 2207 year: 2013 ident: 10.1016/j.freeradbiomed.2013.12.019_bib62 article-title: Aspirin blocks proliferation in colon cells by inducing a G1 arrest and apoptosis through activation of the checkpoint kinase ATM publication-title: Carcinogenesis doi: 10.1093/carcin/bgm101 contributor: fullname: Luciani – volume: 54 start-page: 4308 year: 1994 ident: 10.1016/j.freeradbiomed.2013.12.019_bib32 article-title: Human chromosome 3 corrects mismatch repair deficiency and microsatellite instability and reduces N-methyl-N'-nitro-N-nitrosoguanidine tolerance in colon tumor cells with homozygous hMLH1 mutation publication-title: Cancer Res. contributor: fullname: Koi – volume: 45 start-page: 409 year: 2005 ident: 10.1016/j.freeradbiomed.2013.12.019_bib71 article-title: Oral or intravenous N-acetylcysteine for acetaminophen poisoning publication-title: Ann. Emerg. Med. doi: 10.1016/j.annemergmed.2004.09.028 contributor: fullname: Prescott – volume: 7 start-page: 197 year: 2010 ident: 10.1016/j.freeradbiomed.2013.12.019_bib12 article-title: Mismatch repair deficient colorectal cancer in the era of personalized treatment publication-title: Nat. Rev. Clin. Oncol. doi: 10.1038/nrclinonc.2010.18 contributor: fullname: Hewish – volume: 1 start-page: 881 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib47 article-title: Mutational specificity of mice defective in the MTH1 and/or the MSH2 genes publication-title: DNA Repair doi: 10.1016/S1568-7864(02)00113-1 contributor: fullname: Egashira – volume: 33 start-page: 5094 year: 2005 ident: 10.1016/j.freeradbiomed.2013.12.019_bib50 article-title: 8-Oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSα publication-title: Nucleic Acids Res. doi: 10.1093/nar/gki813 contributor: fullname: Macpherson – volume: 4 start-page: 439 year: 1999 ident: 10.1016/j.freeradbiomed.2013.12.019_bib2 article-title: MSH2 and MSH6 are required for removal of adenine misincorporated opposite 8-oxo-guanine in S. cerevisiae publication-title: Mol. Cell doi: 10.1016/S1097-2765(00)80346-9 contributor: fullname: Ni – volume: 12 start-page: 2137 year: 2007 ident: 10.1016/j.freeradbiomed.2013.12.019_bib42 article-title: Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents publication-title: Nucleic Acids Res doi: 10.1093/nar/12.4.2137 contributor: fullname: Kasai – volume: 25 start-page: 3197 year: 2005 ident: 10.1016/j.freeradbiomed.2013.12.019_bib65 article-title: The influence of high doses of vitamin C and zinc on oxidative DNA damage publication-title: Anticancer Res. contributor: fullname: Harreus – volume: 349 start-page: 431 year: 1991 ident: 10.1016/j.freeradbiomed.2013.12.019_bib43 article-title: Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG publication-title: Nature doi: 10.1038/349431a0 contributor: fullname: Shibutani – volume: 39 start-page: 1131 year: 2010 ident: 10.1016/j.freeradbiomed.2013.12.019_bib70 article-title: Reduction of protein radicals by GSH and ascorbate: potential biological significance publication-title: Amino Acids doi: 10.1007/s00726-010-0610-7 contributor: fullname: Gebicki – volume: 54 start-page: 1645 year: 1994 ident: 10.1016/j.freeradbiomed.2013.12.019_bib6 article-title: Replication errors in benign and malignant tumors from hereditary nonpolyposis colorectal cancer patients publication-title: Cancer Res. contributor: fullname: Aaltonen – volume: 64 start-page: 1307 year: 2004 ident: 10.1016/j.freeradbiomed.2013.12.019_bib25 article-title: Promotion of microsatellite instability by hepatitis C virus core protein in human non-neoplastic hepatocyte cells publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-03-2992 contributor: fullname: Naganuma – volume: 7 start-page: 1686 year: 2009 ident: 10.1016/j.freeradbiomed.2013.12.019_bib52 article-title: Acquired resistance to combination treatment with temozolomide and ABT-888 is mediated by both base excision repair and homologous recombination DNA repair pathways publication-title: Mol. Cancer Res. doi: 10.1158/1541-7786.MCR-09-0299 contributor: fullname: Liu – volume: 1 start-page: 323 year: 2009 ident: 10.1016/j.freeradbiomed.2013.12.019_bib36 article-title: Methotrexate induces oxidative DNA damage and is selectively lethal to tumour cells with defects in the DNA mismatch repair gene MSH2 publication-title: EMBO Mol. Med doi: 10.1002/emmm.200900040 contributor: fullname: Martin – volume: 96 start-page: 261 year: 2004 ident: 10.1016/j.freeradbiomed.2013.12.019_bib41 article-title: Revised Bethesda guidelines for hereditary nonpolyposis colorectal cancer (Lynch syndrome) and microsatellite instability publication-title: J. Natl. Cancer Inst. doi: 10.1093/jnci/djh034 contributor: fullname: Umar – volume: 56 start-page: 1237 year: 1996 ident: 10.1016/j.freeradbiomed.2013.12.019_bib17 article-title: Microsatellite instability in nonneoplastic mucosa from patients with chronic ulcerative colitis publication-title: Cancer Res. contributor: fullname: Brentnall – volume: 93 start-page: 2060 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib18 article-title: Microsatellite instability in non-neoplastic mucosa of patients with ulcerative colitis: effect of folate supplementation publication-title: Am. J. Gastroenterol. doi: 10.1111/j.1572-0241.1998.00592.x contributor: fullname: Cravo – volume: 22 start-page: 1709 year: 2001 ident: 10.1016/j.freeradbiomed.2013.12.019_bib39 article-title: Suppression of spontaneous and hydrogen peroxide-induced mutagenesis by the antioxidant ascorbate in mismatch repair-deficient human colon cancer cells publication-title: Carcinogenesis doi: 10.1093/carcin/22.10.1709 contributor: fullname: Glaab – volume: 82 start-page: 1808 year: 1998 ident: 10.1016/j.freeradbiomed.2013.12.019_bib5 article-title: Microsatellite instability in human solid tumors publication-title: Cancer doi: 10.1002/(SICI)1097-0142(19980515)82:10<1808::AID-CNCR2>3.0.CO;2-J contributor: fullname: Arzimanoglou – volume: 24 start-page: 465 year: 2004 ident: 10.1016/j.freeradbiomed.2013.12.019_bib49 article-title: The oxidized deoxynucleoside triphosphate pool is a significant contributor to genetic instability in mismatch repair-deficient cells publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.24.1.465-474.2004 contributor: fullname: Russo – volume: 283 start-page: C148 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib10 article-title: Oxidative stress inactivates the human DNA mismatch repair system publication-title: Am. J. Physiol. Cell Physiol doi: 10.1152/ajpcell.00422.2001 contributor: fullname: Chang – volume: 277 start-page: 8260 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib45 article-title: Activation of human MutS homologs by 8-oxo-guanine DNA damage publication-title: J. Biol. Chem. doi: 10.1074/jbc.M111269200 contributor: fullname: Mazurek – volume: 57 start-page: 72 year: 2012 ident: 10.1016/j.freeradbiomed.2013.12.019_bib29 article-title: Microsatellite instability, EMAST, and morphology associations with T cell infiltration in colorectal neoplasia publication-title: Dig. Dis. Sci. doi: 10.1007/s10620-011-1825-5 contributor: fullname: Lee – volume: 65 start-page: 3993 year: 2005 ident: 10.1016/j.freeradbiomed.2013.12.019_bib61 article-title: Mesalazine improves replication fidelity in cultured colorectal cells publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-04-3824 contributor: fullname: Gasche – volume: 61 start-page: 7444 year: 2001 ident: 10.1016/j.freeradbiomed.2013.12.019_bib31 article-title: Oxidative stress increases frameshift mutations in human colorectal cancer cells publication-title: Cancer Res. contributor: fullname: Gasche – volume: 67 start-page: 1157 year: 2011 ident: 10.1016/j.freeradbiomed.2013.12.019_bib66 article-title: Ascorbate exerts anti-proliferative effects through cell cycle inhibition and sensitizes tumor cells towards cytostatic drugs publication-title: Cancer Chemother. Pharmacol. doi: 10.1007/s00280-010-1418-6 contributor: fullname: Fromberg – volume: 21 start-page: 1174 year: 2003 ident: 10.1016/j.freeradbiomed.2013.12.019_bib8 article-title: Role of DNA mismatch repair defects in the pathogenesis of human cancer publication-title: J. Clin. Oncol doi: 10.1200/JCO.2003.04.060 contributor: fullname: Peltomaki – volume: 74 start-page: 681 year: 1996 ident: 10.1016/j.freeradbiomed.2013.12.019_bib1 article-title: DNA mismatch repair publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev.biochem.74.082803.133243 contributor: fullname: Kunkel – volume: 29 start-page: 781 year: 2010 ident: 10.1016/j.freeradbiomed.2013.12.019_bib38 article-title: The role of COX-2 in intestinal inflammation and colorectal cancer publication-title: Oncogene doi: 10.1038/onc.2009.421 contributor: fullname: Wang – volume: 112 start-page: 1793 year: 2003 ident: 10.1016/j.freeradbiomed.2013.12.019_bib56 article-title: Tumbling down a different pathway to genetic instability publication-title: J. Clin. Invest doi: 10.1172/JCI200320502 contributor: fullname: Guo – volume: 15 start-page: 2995 year: 2009 ident: 10.1016/j.freeradbiomed.2013.12.019_bib23 article-title: Helicobacter pylori infection induces genetic instability of nuclear and mitochondrial DNA in gastric cells publication-title: Clin. Cancer Res. doi: 10.1158/1078-0432.CCR-08-2686 contributor: fullname: Machado – volume: 277 start-page: 8260 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib3 article-title: Activation of human MutS homologs by 8-oxo-guanine DNA damage publication-title: J. Biol. Chem. doi: 10.1074/jbc.M111269200 contributor: fullname: Mazurek – volume: 277 start-page: 11135 year: 2002 ident: 10.1016/j.freeradbiomed.2013.12.019_bib46 article-title: Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M108618200 contributor: fullname: Gu |
SSID | ssj0004538 |
Score | 2.1694133 |
Snippet | The DNA mismatch-repair (MMR) system corrects replicative errors and minimizes mutations that occur at a high rate in microsatellites. Patients with chronic... |
SourceID | proquest crossref pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 86 |
SubjectTerms | DNA Mismatch Repair - drug effects DNA-Binding Proteins - genetics Fluorescent Dyes - chemistry Genes, Reporter Humans Hydrogen Peroxide - toxicity Inflammation - chemically induced Inflammation - drug therapy Inflammation - genetics Microsatellite Instability - drug effects Mutation Neoplasms - drug therapy Neoplasms - genetics Neoplasms - pathology Oxidative Stress Sulfhydryl Compounds - chemistry Sulfhydryl Compounds - isolation & purification Sulfhydryl Compounds - pharmacology |
Title | A dual-fluorescent reporter facilitates identification of thiol compounds that suppress microsatellite instability induced by oxidative stress |
URI | https://www.ncbi.nlm.nih.gov/pubmed/24412704 https://search.proquest.com/docview/1508681869 |
Volume | 69 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFLWqIRAvCDZg40tGIF4qV03ifD1WFdNAE0-b2J4ix3Zopi2Z0kSiPPAT-D_8O-61kzQtTBp7iRq3dZLe0-tr-9xzCXkPKJE4rjEx9SXjkZAsCnyHeSLiPA79eKoM2-JLcHTKP5_5Z6PR7wFrqanTifzxz7ySu1gV2sCumCX7H5btO4UGeA32hSNYGI63svFsjJlULLtsysrKMrV7ALrCQjpWgBsZV6rlBPXxYb3Iy0vDJ8eySks4F_V42VwbWuz4Cll6S2HEOmtUFYEQ0pBoMUlQNdJGreX3XFnZcJtwMoxzDyutx5Wwm0Cd0BOibHsv_9iwCT6x-WKdkzZf5I2lAoDfYefNGsBf23ZRfGPH-fALBo2LfMHOu7G4XcpwhgyY1uPFDgPkxEP3bCu5tP61k802J7Y6519jgF2OuJhk8KTIRzAyBkji88zCb-uhN5S3t0bEnqfYUeAuko3OEuwscdxkioKz99ww9pFNOvnpDITqTRH1_okekHdrauGNd7YZHN0w4zGRz8lj8qidstCZxd8TMtLFLtmbFaIur1b0AzUkYmPhXXLf1jZd7ZFfM7oNTtqBkw7ASTfBScuMGnDSHpwUwUk7cNJNcNIBOGkLTpquaA9OasH5lJwefjyZH7G2-AeTnuvULMMt_IxzT6ZSu16oAgVTBz8Nw0zBO6EI4-lUKSnTKOWOE-sAAk-YXQQQkvnKS71nZKcoC71PKAxBQrpcBxnEYpEMI8WzmCvkT3NXaeeA8O5XT66txktyC8sfkLedhRLwybjRJgpdNssEaywEKBUJn3luTdd3DNdEsgd_cbeLviQP1_-cV2Snrhr9GsLiOn1jAPgH6_XBcQ |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Elsevier |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+dual-fluorescent+reporter+facilitates+identification+of+thiol+compounds+that+suppress+microsatellite+instability+induced+by+oxidative+stress&rft.jtitle=Free+radical+biology+%26+medicine&rft.au=Li%2C+I-Chen&rft.au=Chiu%2C+Chien-Yuan&rft.au=Wu%2C+Chang-Lin&rft.au=Chi%2C+Jhih-Ying&rft.date=2014-04-01&rft.issn=0891-5849&rft.volume=69&rft.spage=86&rft.epage=95&rft_id=info:doi/10.1016%2Fj.freeradbiomed.2013.12.019&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_freeradbiomed_2013_12_019 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0891-5849&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0891-5849&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0891-5849&client=summon |