Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate

The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enz...

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Bibliographic Details
Published inThe Korean journal of chemical engineering Vol. 26; no. 1; pp. 127 - 130
Main Authors Ponvel, Kanagasabai Muruganandam, Lee, Dong-Geun, Woo, Eun-Ji, Ahn, Ik-Sung, Lee, Chang-Ha
Format Journal Article
LanguageEnglish
Published Boston Springer US 2009
한국화학공학회
Subjects
Biotechnology
Catalysis
Chemistry
Chemistry and Materials Science
Environmental Engineering
Industrial Chemistry/Chemical Engineering
Materials Science
화학공학
Enzyme Immobilization
Nano-sized Magnetite
Alkyl Benzenesulfonate
Lipase
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Summary:The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.
Bibliography:http://www.cheric.org/article/724506
G704-000406.2009.26.1.018
ISSN:0256-1115
1975-7220
DOI:10.1007/s11814-009-0019-8