Components in Melanoma Cytoplasm Might Induce Murine BMSCs Transformation and Expression of Melan-A

This study explored the possibility that the components in melanoma cytoplasm induce murine BMSCs transformation and expression of Melan-A by morphologically observing the changes of BMSCs and immunocytochemically detecting Melan-A in the cells after culturing BMSCs in me-dium containing melanoma cy...

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Published inJournal of Huazhong University of Science and Technology. Medical sciences Vol. 31; no. 5; pp. 663 - 666
Main Author 麻婧 史俊娟 王建超 刘俊 吴轲 敖启林 刘卓雅 王小丽 刘胜洪
Format Journal Article
LanguageEnglish
Published Heidelberg Huazhong University of Science and Technology 01.10.2011
Subjects
Animals
Bone Marrow Cells - cytology
Cell Line, Tumor
Cell Transformation, Neoplastic - metabolism
Cell Transformation, Neoplastic - pathology
Coculture Techniques
Cytoplasm - chemistry
Humans
Male
MART-1 Antigen - metabolism
Medicine
Medicine & Public Health
Melanoma, Experimental - chemistry
Melanoma, Experimental - pathology
Mice
Skin Neoplasms - chemistry
Skin Neoplasms - pathology
Stem Cells - cytology
元件
小鼠
细胞质
细胞转化
诱发
诱导培养基
骨髓基质干细胞
黑色素瘤
melanogenic cells
melanoma
BMSCs
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ISSN1672-0733
1993-1352
DOI10.1007/s11596-011-0578-x

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Summary:This study explored the possibility that the components in melanoma cytoplasm induce murine BMSCs transformation and expression of Melan-A by morphologically observing the changes of BMSCs and immunocytochemically detecting Melan-A in the cells after culturing BMSCs in me-dium containing melanoma cytoplasm components （MCC）. MCC of B16 melanoma cells was prepared and BMSCs were cultured and induced by adding the MCC into culture medium. The cells were morphologically observed and Melan-A was immunohistochemically detected to confirm BMSCs transformation. MCC-induced BMSCs underwent morphological changes. A number of melanin granules appeared in the cytoplasm of the cells and some were released into surrounding areas. Several cells that might come from one cell formed a cluster, and their granules, together with those secreted by other induced BMSCs, formed a so-called ＂sphere-formed structure＂. The induced BMSCs expressed Melan-A. We are led to conclude that there might be some factors in the cytoplasm of melanoma cells that might induce BMSCs transformation toward melanogenic cell, or even melanoma.
Bibliography:42-1679/R
BMSCs melanogenic cells melanoma
This study explored the possibility that the components in melanoma cytoplasm induce murine BMSCs transformation and expression of Melan-A by morphologically observing the changes of BMSCs and immunocytochemically detecting Melan-A in the cells after culturing BMSCs in me-dium containing melanoma cytoplasm components （MCC）. MCC of B16 melanoma cells was prepared and BMSCs were cultured and induced by adding the MCC into culture medium. The cells were morphologically observed and Melan-A was immunohistochemically detected to confirm BMSCs transformation. MCC-induced BMSCs underwent morphological changes. A number of melanin granules appeared in the cytoplasm of the cells and some were released into surrounding areas. Several cells that might come from one cell formed a cluster, and their granules, together with those secreted by other induced BMSCs, formed a so-called ＂sphere-formed structure＂. The induced BMSCs expressed Melan-A. We are led to conclude that there might be some factors in the cytoplasm of melanoma cells that might induce BMSCs transformation toward melanogenic cell, or even melanoma.
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ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-011-0578-x