Isolation, identification and characterization of Shewanella algae from reared tongue sole, Cynoglossus semilaevis Günther
In this study, a Gram-negative bacterium was isolated from diseased Cynoglossus semilaevis (C. semilaevis) and tentatively named strain CSG-15. It was preliminary identified as Shewanella algae (S. algae) based on morphological, physiological and biochemical characteristics. The sequenced 16S rRNA g...
Saved in:
Published in | Aquaculture Vol. 468; pp. 356 - 362 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.02.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | In this study, a Gram-negative bacterium was isolated from diseased Cynoglossus semilaevis (C. semilaevis) and tentatively named strain CSG-15. It was preliminary identified as Shewanella algae (S. algae) based on morphological, physiological and biochemical characteristics. The sequenced 16S rRNA gene of CSG-15 strain (Genbank accession no. KX455851) was 1509bp. And it exhibited 99.86%, 99.86% and 99.80% of identities compared with those of S. algae strains ATCC51192 (NR117771), YJ06114 (EF542799), and MAS2737 (GQ372875), respectively. Phylogenetic analysis grouped CSG-15 strain in the S. algae cluster, and showed the closest relation to S. algae ATCC51192 and YJ06114. Infection experiment showed that the median lethal dosage (LD50) for CSG-15 was calculated as 1.0×106CFU/g fish weight. The pathological observation on the intestine showed that CSG-15 strain caused noticeable histological lesions, such as inflammatory cell infiltrating and villi shedding. Antimicrobial susceptibility test showed that it was highly sensitive to most of aminoglycosides, β-lactams, tetracyclines, quinolones, macrolides aminocyclitol, sulphonamides, chloramphenicols antibiotic. Growing characteristics showed that CSG-15 could grow under the condition of temperature 26–34°C, pH5–9 and 20–50ppt, and had a better adaptability to environment. The growth curve of strain CSG-15 under the condition of 30°C, pH7.0 and 30ppt showed four distinct phases of bacterial growth. To the best of our knowledge, this is the first report of S. algae linked to diseased C. semilaevis.
In this study, a new Shewanella algae strain was isolated from diseased C. semilaevis for the first time. It was identified based on morphological, physiological, biochemical characteristics and 16S rRNA gene sequences analysis. Subsequently, antimicrobial susceptibility, pathogenicity and growing characteristics of S. algae were studied. This report can provide a scientific reference for characterization of Shewanella species, prevention and treatment of bacterial disease caused by S. algae in fish.
•A S. algae was isolated from diseased C. semilaevis for the first time.•S. algae caused noticeable histological lesions on the intestine.•The LD50 was calculated as 1.0×106CFU/g fish weight of C. semilaevis.•S. algae was highly sensitive to most of β-lactams, tetracyclines antibiotic and so on.•S. algae could grow under the condition of temperature 26–34°C, pH5–9 and 20–50ppt. |
---|---|
AbstractList | In this study, a Gram-negative bacterium was isolated from diseased Cynoglossus semilaevis (C. semilaevis) and tentatively named strain CSG-15. It was preliminary identified as Shewanella algae (S. algae) based on morphological, physiological and biochemical characteristics. The sequenced 16S rRNA gene of CSG-15 strain (Genbank accession no. KX455851) was 1509bp. And it exhibited 99.86%, 99.86% and 99.80% of identities compared with those of S. algae strains ATCC51192 (NR117771), YJ06114 (EF542799), and MAS2737 (GQ372875), respectively. Phylogenetic analysis grouped CSG-15 strain in the S. algae cluster, and showed the closest relation to S. algae ATCC51192 and YJ06114. Infection experiment showed that the median lethal dosage (LD50) for CSG-15 was calculated as 1.0×106CFU/g fish weight. The pathological observation on the intestine showed that CSG-15 strain caused noticeable histological lesions, such as inflammatory cell infiltrating and villi shedding. Antimicrobial susceptibility test showed that it was highly sensitive to most of aminoglycosides, β-lactams, tetracyclines, quinolones, macrolides aminocyclitol, sulphonamides, chloramphenicols antibiotic. Growing characteristics showed that CSG-15 could grow under the condition of temperature 26–34°C, pH5–9 and 20–50ppt, and had a better adaptability to environment. The growth curve of strain CSG-15 under the condition of 30°C, pH7.0 and 30ppt showed four distinct phases of bacterial growth. To the best of our knowledge, this is the first report of S. algae linked to diseased C. semilaevis.
In this study, a new Shewanella algae strain was isolated from diseased C. semilaevis for the first time. It was identified based on morphological, physiological, biochemical characteristics and 16S rRNA gene sequences analysis. Subsequently, antimicrobial susceptibility, pathogenicity and growing characteristics of S. algae were studied. This report can provide a scientific reference for characterization of Shewanella species, prevention and treatment of bacterial disease caused by S. algae in fish.
•A S. algae was isolated from diseased C. semilaevis for the first time.•S. algae caused noticeable histological lesions on the intestine.•The LD50 was calculated as 1.0×106CFU/g fish weight of C. semilaevis.•S. algae was highly sensitive to most of β-lactams, tetracyclines antibiotic and so on.•S. algae could grow under the condition of temperature 26–34°C, pH5–9 and 20–50ppt. |
Author | Sun, Jingfeng Lv, Aijun Shi, Hongyue Sung, YeongYik Xing, Kezhi Hu, Xiucai Han, Zhuoran |
Author_xml | – sequence: 1 givenname: Zhuoran surname: Han fullname: Han, Zhuoran – sequence: 2 givenname: Jingfeng surname: Sun fullname: Sun, Jingfeng email: sun_jf@163.com – sequence: 3 givenname: Aijun surname: Lv fullname: Lv, Aijun – sequence: 4 givenname: YeongYik surname: Sung fullname: Sung, YeongYik – sequence: 5 givenname: Hongyue surname: Shi fullname: Shi, Hongyue – sequence: 6 givenname: Xiucai surname: Hu fullname: Hu, Xiucai – sequence: 7 givenname: Kezhi surname: Xing fullname: Xing, Kezhi |
BookMark | eNqNkMtOwzAQRS1UJNrCP5h9E-w4cZMlqnhUqsQCWEdTe9y6Sm2wk6LCr7Hjx0gpC5asRnNG92p0RmTgvENCLjlLOePyapPCaweqa9ouYJr1qOcpE-UJGfJyKpJCZtmADBnL86TMS3lGRjFuGGNSFnxIPubRN9Ba7ybUanStNVb97BScpmoNAVSLwb4foTf0cY1v4LBpgEKzAqQm-C0NCAE1bb1bdUj7UpzQ2d75VeNj7CKNuLUN4M5Gevf16do1hnNyaqCJePE7x-T59uZpdp8sHu7ms-tFokTG20QaqfOp4QA8E8jzPCshL6YaS1QgiiVb9ryqpGKaS6HLUpnKaFyyQugMcyHGpDr2qtD_EtDUL8FuIexrzuqDxXpT_7FYHyweTr3FPjs7ZrF_cGcx1FFZdAq1DajaWnv7j5ZvZUCH9g |
CitedBy_id | crossref_primary_10_1007_s10163_023_01794_6 crossref_primary_10_1016_j_fsi_2019_08_059 crossref_primary_10_1016_j_fsi_2018_06_007 crossref_primary_10_1016_j_micpath_2022_105662 crossref_primary_10_1007_s11259_020_09773_0 crossref_primary_10_3354_dao03665 crossref_primary_10_1016_j_aqrep_2022_101292 crossref_primary_10_1016_j_aquaculture_2021_737614 crossref_primary_10_1155_2019_3840563 crossref_primary_10_1016_j_aqrep_2021_100758 crossref_primary_10_1080_15567036_2023_2232322 crossref_primary_10_3354_dao03363 crossref_primary_10_3389_fimmu_2022_900793 crossref_primary_10_14202_vetworld_2021_678_688 crossref_primary_10_1155_2020_9205197 crossref_primary_10_1016_j_aquaculture_2018_01_027 crossref_primary_10_1007_s11831_023_09950_9 crossref_primary_10_3390_microorganisms10030572 crossref_primary_10_1007_s10482_020_01444_z crossref_primary_10_1016_j_aquaculture_2024_741286 crossref_primary_10_1016_j_fsi_2020_06_013 crossref_primary_10_1016_j_ijbiomac_2023_127040 crossref_primary_10_1016_j_aqrep_2022_101293 crossref_primary_10_1155_2018_6976897 crossref_primary_10_1111_jwas_13000 crossref_primary_10_1016_j_fsi_2020_09_020 crossref_primary_10_1016_j_aquaculture_2018_09_051 crossref_primary_10_1016_j_cimid_2020_101545 crossref_primary_10_1016_j_aquaculture_2021_736779 crossref_primary_10_1099_mgen_0_000549 crossref_primary_10_1016_j_fsi_2024_109518 crossref_primary_10_1080_15435075_2023_2255647 crossref_primary_10_1016_j_watbs_2024_100277 crossref_primary_10_3389_fvets_2020_600587 crossref_primary_10_2139_ssrn_4177782 crossref_primary_10_1186_s13568_020_00973_0 crossref_primary_10_3390_microorganisms12030503 crossref_primary_10_1016_j_aquaculture_2019_734208 crossref_primary_10_3389_fmicb_2019_02742 crossref_primary_10_1016_j_aquaculture_2023_739813 crossref_primary_10_1016_j_aquaculture_2018_02_036 crossref_primary_10_1002_aah_10141 crossref_primary_10_1128_mSystems_00917_21 crossref_primary_10_1016_j_heliyon_2023_e17775 |
Cites_doi | 10.1099/ijs.0.02997-0 10.1111/jfd.12231 10.1021/es60140a017 10.1099/ijs.0.64708-0 10.1128/AEM.63.6.2189-2199.1997 10.1021/es201608q 10.1128/JCM.36.3.783-787.1998 10.1099/ijs.0.64500-0 10.1007/s10499-010-9319-6 10.1128/IAI.56.12.3285-3293.1988 10.1016/j.jhsa.2016.04.012 10.3947/ic.2014.46.4.264 10.1163/156855902760262808 10.1007/s10152-014-0386-3 10.2169/internalmedicine.52.8152 10.21767/AMJ.2015.2292 10.1099/00207713-40-4-331 10.1007/s10072-014-1956-5 10.3724/SP.J.1231.2012.27667 10.1128/AEM.65.9.3896-3900.1999 10.1111/j.1469-0691.2005.01108.x |
ContentType | Journal Article |
Copyright | 2016 Elsevier B.V. |
Copyright_xml | – notice: 2016 Elsevier B.V. |
DBID | AAYXX CITATION |
DOI | 10.1016/j.aquaculture.2016.10.038 |
DatabaseName | CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Agriculture |
EISSN | 1873-5622 |
EndPage | 362 |
ExternalDocumentID | 10_1016_j_aquaculture_2016_10_038 S004484861630816X |
GroupedDBID | --K --M -~X .~1 0R~ 1B1 1RT 1~. 1~5 23M 4.4 457 4G. 5GY 5VS 6J9 7-5 71M 8P~ 9JM AABNK AABVA AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AATLK AAXUO ABBQC ABFNM ABFRF ABGRD ABJNI ABKYH ABLVK ABMAC ABMZM ABRWV ABYKQ ACDAQ ACGFO ACGFS ACIUM ACIWK ACPRK ACRLP ADBBV ADEZE ADQTV AEBSH AEFWE AEKER AENEX AEQOU AESVU AEXOQ AFKWA AFRAH AFTJW AFXIZ AGHFR AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX AXJTR BKOJK BKOMP BLXMC BNPGV CBWCG CS3 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FYGXN G-Q GBLVA IHE J1W KOM LCYCR LW9 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. PQQKQ Q38 QYZTP RIG ROL RPZ RSU SAB SCC SDF SDG SES SNL SPCBC SSA SSH SSZ T5K WH7 ~G- ~KM AAHBH AALCJ AAXKI AAYJJ AAYXX ABXDB AFJKZ AKRWK ASPBG AVWKF AZFZN CITATION FEDTE FGOYB G-2 HLV HVGLF HZ~ R2- SEW WUQ Y6R |
ID | FETCH-LOGICAL-c321t-6f6d47f1aa123e14428a457de8eca35b0b123996c0d163d88cf9fdeb053d2e433 |
IEDL.DBID | AIKHN |
ISSN | 0044-8486 |
IngestDate | Thu Sep 26 17:57:47 EDT 2024 Fri Feb 23 02:31:40 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | Antimicrobial susceptibility Cynoglossus semilaevis Shewanella algae 16S rDNA |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c321t-6f6d47f1aa123e14428a457de8eca35b0b123996c0d163d88cf9fdeb053d2e433 |
PageCount | 7 |
ParticipantIDs | crossref_primary_10_1016_j_aquaculture_2016_10_038 elsevier_sciencedirect_doi_10_1016_j_aquaculture_2016_10_038 |
PublicationCentury | 2000 |
PublicationDate | 2017-02-01 2017-02-00 |
PublicationDateYYYYMMDD | 2017-02-01 |
PublicationDate_xml | – month: 02 year: 2017 text: 2017-02-01 day: 01 |
PublicationDecade | 2010 |
PublicationTitle | Aquaculture |
PublicationYear | 2017 |
Publisher | Elsevier B.V |
Publisher_xml | – name: Elsevier B.V |
References | Yang, Guo, Xu, Zhang, Wang, Jing (bb0150) 2009; 25 Yue, Liu, Deng, Gong (bb0155) 2007; 17 Zhang, Qin, Yan, Xu, Bi, Qin (bb0165) 2009; 31 Zadeh, Saad, Christianus, Kamarudin, Sijam, Shamsudin, Neela (bb0160) 2010; 18 Khashe, Janda (bb0065) 1998; 36 Charles, Srirangaraj, Kali (bb0015) 2015; 8 Love, Rodman, Neff, Nachman (bb0090) 2011; 45 Ivanova, Flavier, Christen (bb0060) 2004; 54 Hamilton, Russo, Thurston (bb0045) 1978; 12 Krsnik, Arribalzaga, Romanyk (bb0075) 2002; 32 Simidu, Kitatsukamoto, Yasumoto, Yotsu (bb0115) 1990; 40 Gram, Bundvad, Melchiorsen, Johansen, Fonnesbech (bb0040) 1999; 65 Chen, Hu, Chen, Zhang (bb0020) 2003; 34 Pękala, Kozińska, Paździor, Głowacka (bb0095) 2014; 38 Cao, Yong-Wen, Lei, Jiang, Heng (bb0010) 2007; 47 Duan, Wang, Wang, Xiao, Han, Zhang (bb0030) 2015; 36 Liu, Lin, Tung, Shyu, Wu, Liu, Chang, Chan, Huang, Shi (bb0085) 2013; 52 Fluke, Carayannopoulos, Lindsey (bb0035) 2016; 41 Zhang, Peng, Zhang, Liu, Fu, Luo (bb0170) 2010; 50 Satomi, Vogel, Venkateswaran, Gram (bb0110) 2007; 57 Wang, Zhang, Bai, Chen, Guo, Xing (bb0140) 2015; 332 Ariole, Eddo (bb0005) 2015; 5 Chen, Yao, Lin, Chang (bb0025) 2012; 36 Zhang, Zhu, Wang (bb0175) 2013; 26 Vogel, Jørgensen, Christensen, Olsen, Gram (bb0130) 1997; 63 Wang, Han, Yun, Chen, Zhang (bb0135) 2007; 47 Rachanamol, Lipton, Thankamani, Sarika, Selvin (bb0100) 2014; 68 Li, Zhang, Qiu, Yang, Huang (bb0080) 2015; 16 Hu, Sun, Chen, Xing (bb0055) 2014; 21 Xiao, Wang, Zeng, Bartlett, Wang (bb0145) 2007; 57 Holt, Gahrn-Hansen, Bruun (bb0050) 2005; 11 Telke, Rolain (bb0125) 2015; 36 Kim, Cho, Kang, Kim, Byun, Park, Choi (bb0070) 2014; 46 Santos, Toranzo, Barja, Nieto, Villa (bb0105) 1988; 56 Sivolodskiĭ, Kotiv, Kolobova, Gorelova, Bogoslovskaia, Barinov, Ivanov (bb0120) 2012; 74-76 Zadeh (10.1016/j.aquaculture.2016.10.038_bb0160) 2010; 18 Krsnik (10.1016/j.aquaculture.2016.10.038_bb0075) 2002; 32 Pękala (10.1016/j.aquaculture.2016.10.038_bb0095) 2014; 38 Love (10.1016/j.aquaculture.2016.10.038_bb0090) 2011; 45 Ariole (10.1016/j.aquaculture.2016.10.038_bb0005) 2015; 5 Fluke (10.1016/j.aquaculture.2016.10.038_bb0035) 2016; 41 Wang (10.1016/j.aquaculture.2016.10.038_bb0140) 2015; 332 Wang (10.1016/j.aquaculture.2016.10.038_bb0135) 2007; 47 Hu (10.1016/j.aquaculture.2016.10.038_bb0055) 2014; 21 Liu (10.1016/j.aquaculture.2016.10.038_bb0085) 2013; 52 Hamilton (10.1016/j.aquaculture.2016.10.038_bb0045) 1978; 12 Satomi (10.1016/j.aquaculture.2016.10.038_bb0110) 2007; 57 Yang (10.1016/j.aquaculture.2016.10.038_bb0150) 2009; 25 Zhang (10.1016/j.aquaculture.2016.10.038_bb0165) 2009; 31 Zhang (10.1016/j.aquaculture.2016.10.038_bb0175) 2013; 26 Li (10.1016/j.aquaculture.2016.10.038_bb0080) 2015; 16 Rachanamol (10.1016/j.aquaculture.2016.10.038_bb0100) 2014; 68 Cao (10.1016/j.aquaculture.2016.10.038_bb0010) 2007; 47 Holt (10.1016/j.aquaculture.2016.10.038_bb0050) 2005; 11 Santos (10.1016/j.aquaculture.2016.10.038_bb0105) 1988; 56 Yue (10.1016/j.aquaculture.2016.10.038_bb0155) 2007; 17 Vogel (10.1016/j.aquaculture.2016.10.038_bb0130) 1997; 63 Sivolodskiĭ (10.1016/j.aquaculture.2016.10.038_bb0120) 2012; 74-76 Gram (10.1016/j.aquaculture.2016.10.038_bb0040) 1999; 65 Telke (10.1016/j.aquaculture.2016.10.038_bb0125) 2015; 36 Khashe (10.1016/j.aquaculture.2016.10.038_bb0065) 1998; 36 Kim (10.1016/j.aquaculture.2016.10.038_bb0070) 2014; 46 Zhang (10.1016/j.aquaculture.2016.10.038_bb0170) 2010; 50 Simidu (10.1016/j.aquaculture.2016.10.038_bb0115) 1990; 40 Duan (10.1016/j.aquaculture.2016.10.038_bb0030) 2015; 36 Xiao (10.1016/j.aquaculture.2016.10.038_bb0145) 2007; 57 Charles (10.1016/j.aquaculture.2016.10.038_bb0015) 2015; 8 Chen (10.1016/j.aquaculture.2016.10.038_bb0020) 2003; 34 Ivanova (10.1016/j.aquaculture.2016.10.038_bb0060) 2004; 54 Chen (10.1016/j.aquaculture.2016.10.038_bb0025) 2012; 36 |
References_xml | – volume: 18 start-page: 1017 year: 2010 end-page: 1026 ident: bb0160 article-title: Assessment of growth condition for a candidate probiotic, publication-title: Aquac. Int. contributor: fullname: Neela – volume: 52 start-page: 431 year: 2013 end-page: 438 ident: bb0085 article-title: Clinical and microbiological features of Shewanella bacteremia in patients with hepatobiliary disease publication-title: J. Intern. Med. contributor: fullname: Shi – volume: 34 start-page: 1 year: 2003 end-page: 8 ident: bb0020 article-title: Identification and characterization of publication-title: Oceanologia. Limnologia. Sinica. contributor: fullname: Zhang – volume: 21 start-page: 12 year: 2014 end-page: 16 ident: bb0055 article-title: Isolation and identification of pathogen of ascites disease from publication-title: J. Tianjin. Agric. Coll. contributor: fullname: Xing – volume: 74-76 year: 2012 ident: bb0120 article-title: Isolation of publication-title: Zh. Mikrobiol. Epidemiol. Immunobiol. contributor: fullname: Ivanov – volume: 45 start-page: 7232 year: 2011 end-page: 7240 ident: bb0090 article-title: Veterinary drug residues in seafood inspected by the European Union, United States, Canada, and Japan from 2000 to 2009 publication-title: Environ. Sci. Technol. contributor: fullname: Nachman – volume: 47 start-page: 1 year: 2007 end-page: 6 ident: bb0010 article-title: Isolation, identification, phylogenetic analysis and related properties of a pathogen in publication-title: Acta Microbiol Sin. contributor: fullname: Heng – volume: 12 start-page: 714 year: 1978 end-page: 719 ident: bb0045 article-title: Trimmed spearman-karber method for estimating median lethal concentrations in toxicity bioassays publication-title: Environ. Sci. Technol. contributor: fullname: Thurston – volume: 54 start-page: 1773 year: 2004 end-page: 1788 ident: bb0060 article-title: Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov publication-title: Int. J. Syst. Evol. Microbiol. contributor: fullname: Christen – volume: 17 start-page: 63 year: 2007 end-page: 66 ident: bb0155 article-title: Choice of optimal culture medium on Shewanella alga publication-title: Biotechnology contributor: fullname: Gong – volume: 31 start-page: 112 year: 2009 end-page: 122 ident: bb0165 article-title: Phenotypic and molecular characterization of pathogenic publication-title: Acta Oceanol. Sin. contributor: fullname: Qin – volume: 56 start-page: 3285 year: 1988 end-page: 3293 ident: bb0105 article-title: Virulence properties and enterotoxin production of Aeromonas strains isolated from fish publication-title: Infect. Immun. contributor: fullname: Villa – volume: 36 start-page: 764 year: 2012 end-page: 771 ident: bb0025 article-title: Study on pathogen of skin ulcer disease of half-smooth tongue sole ( publication-title: J. Fish. China contributor: fullname: Chang – volume: 16 start-page: 1859 year: 2015 ident: bb0080 article-title: Biological characteristics and pathogenicities of publication-title: Agric. Sci. Technol. contributor: fullname: Huang – volume: 332 start-page: 245 year: 2015 end-page: 255 ident: bb0140 article-title: Estimation of dietary copper (Cu) requirement of publication-title: LNEE. contributor: fullname: Xing – volume: 47 start-page: 763 year: 2007 end-page: 768 ident: bb0135 article-title: Isolation of photobacterium damselae subsp. piscicida from diseased tongue sole ( publication-title: Acta Microbiol Sin. contributor: fullname: Zhang – volume: 57 start-page: 60 year: 2007 end-page: 65 ident: bb0145 article-title: sp. nov. and publication-title: Int. J. Syst. Evol. Microbiol. contributor: fullname: Wang – volume: 41 start-page: 203 year: 2016 end-page: 206 ident: bb0035 article-title: Pyogenic flexor tenosynovitis caused by publication-title: J. Hand. Surg. contributor: fullname: Lindsey – volume: 57 start-page: 347 year: 2007 end-page: 352 ident: bb0110 article-title: Description of publication-title: Int. J. Syst. Bacteriol. contributor: fullname: Gram – volume: 11 start-page: 347 year: 2005 end-page: 352 ident: bb0050 article-title: and publication-title: Clin. Microbiol. Infect. contributor: fullname: Bruun – volume: 40 start-page: 331 year: 1990 end-page: 336 ident: bb0115 article-title: Taxonomy of four marine bacterial strains that produce tetrodotoxin publication-title: Int. J. Syst. Bacteriol. contributor: fullname: Yotsu – volume: 25 start-page: 699 year: 2009 end-page: 700 ident: bb0150 article-title: Study on biological characteristics of Shewanella publication-title: Chin. J. Zoonoses. contributor: fullname: Jing – volume: 26 start-page: 6 year: 2013 end-page: 11 ident: bb0175 article-title: Pathogeny and pathogenicity of kidney intumesce of publication-title: J. Ningbo. Univ. contributor: fullname: Wang – volume: 8 start-page: 64 year: 2015 end-page: 66 ident: bb0015 article-title: Neonatal sepsis caused by publication-title: Australas. Med. J. contributor: fullname: Kali – volume: 46 start-page: 264 year: 2014 end-page: 268 ident: bb0070 article-title: A case of spontaneous bacterial peritonitis with bacteremia caused by publication-title: Infect. Chemother. contributor: fullname: Choi – volume: 63 start-page: 2189 year: 1997 end-page: 2199 ident: bb0130 article-title: Differentiation of publication-title: Appl. Environ. Microbiol. contributor: fullname: Gram – volume: 5 start-page: 1 year: 2015 end-page: 9 ident: bb0005 article-title: Effect of an indigenous probiotic ( publication-title: Int. J. Aquacult. contributor: fullname: Eddo – volume: 36 start-page: 783 year: 1998 end-page: 787 ident: bb0065 article-title: Biochemical and pathogenic properties of publication-title: J. Clin. Microbiol. contributor: fullname: Janda – volume: 36 start-page: 1 year: 2015 end-page: 5 ident: bb0125 article-title: Functional genomics to discover antibiotic resistance genes: the paradigm of resistance to colistin mediated by ethanolamine phosphotransferase in publication-title: Int. J. Antimicrob. Agents contributor: fullname: Rolain – volume: 38 start-page: 283 year: 2014 end-page: 293 ident: bb0095 article-title: Phenotypical and genotypical characterization of publication-title: J. Fish Dis. contributor: fullname: Głowacka – volume: 50 start-page: 304 year: 2010 end-page: 309 ident: bb0170 article-title: Isolation and identification of the pathogenic strain of publication-title: Acta Microbiol Sin. contributor: fullname: Luo – volume: 36 start-page: 625 year: 2015 end-page: 629 ident: bb0030 article-title: A case report of intracranial infection caused by publication-title: Neurol. Sci. contributor: fullname: Zhang – volume: 65 start-page: 3896 year: 1999 end-page: 3900 ident: bb0040 article-title: Occurrence of publication-title: Appl. Environ. Microbiol. contributor: fullname: Fonnesbech – volume: 32 start-page: 79 year: 2002 end-page: 80 ident: bb0075 article-title: Shewanella alga bacteremia and associated cellulitis in a patient with multiple myeloma publication-title: Haematologia contributor: fullname: Romanyk – volume: 68 start-page: 263 year: 2014 end-page: 269 ident: bb0100 article-title: Molecular characterization and bioactivity profile of the tropical sponge-associated bacterium publication-title: Helgol. Mar. Res. contributor: fullname: Selvin – volume: 25 start-page: 699 year: 2009 ident: 10.1016/j.aquaculture.2016.10.038_bb0150 article-title: Study on biological characteristics of Shewanella publication-title: Chin. J. Zoonoses. contributor: fullname: Yang – volume: 54 start-page: 1773 year: 2004 ident: 10.1016/j.aquaculture.2016.10.038_bb0060 publication-title: Int. J. Syst. Evol. Microbiol. doi: 10.1099/ijs.0.02997-0 contributor: fullname: Ivanova – volume: 38 start-page: 283 year: 2014 ident: 10.1016/j.aquaculture.2016.10.038_bb0095 article-title: Phenotypical and genotypical characterization of Shewanella putrefaciens, strains isolated from diseased freshwater fish publication-title: J. Fish Dis. doi: 10.1111/jfd.12231 contributor: fullname: Pękala – volume: 12 start-page: 714 year: 1978 ident: 10.1016/j.aquaculture.2016.10.038_bb0045 article-title: Trimmed spearman-karber method for estimating median lethal concentrations in toxicity bioassays publication-title: Environ. Sci. Technol. doi: 10.1021/es60140a017 contributor: fullname: Hamilton – volume: 57 start-page: 347 year: 2007 ident: 10.1016/j.aquaculture.2016.10.038_bb0110 article-title: Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana publication-title: Int. J. Syst. Bacteriol. doi: 10.1099/ijs.0.64708-0 contributor: fullname: Satomi – volume: 5 start-page: 1 year: 2015 ident: 10.1016/j.aquaculture.2016.10.038_bb0005 article-title: Effect of an indigenous probiotic (Shewanella algae) isolated from healthy shrimp (Penaeus monodon) intestine on Clarias gariepinus publication-title: Int. J. Aquacult. contributor: fullname: Ariole – volume: 63 start-page: 2189 year: 1997 ident: 10.1016/j.aquaculture.2016.10.038_bb0130 article-title: Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16s rRNA gene sequence analysis publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.63.6.2189-2199.1997 contributor: fullname: Vogel – volume: 45 start-page: 7232 year: 2011 ident: 10.1016/j.aquaculture.2016.10.038_bb0090 article-title: Veterinary drug residues in seafood inspected by the European Union, United States, Canada, and Japan from 2000 to 2009 publication-title: Environ. Sci. Technol. doi: 10.1021/es201608q contributor: fullname: Love – volume: 26 start-page: 6 year: 2013 ident: 10.1016/j.aquaculture.2016.10.038_bb0175 article-title: Pathogeny and pathogenicity of kidney intumesce of Sciaenops ocellatus publication-title: J. Ningbo. Univ. contributor: fullname: Zhang – volume: 47 start-page: 763 year: 2007 ident: 10.1016/j.aquaculture.2016.10.038_bb0135 article-title: Isolation of photobacterium damselae subsp. piscicida from diseased tongue sole (Cynoglossus semilaevis Günther) in China publication-title: Acta Microbiol Sin. contributor: fullname: Wang – volume: 36 start-page: 783 year: 1998 ident: 10.1016/j.aquaculture.2016.10.038_bb0065 article-title: Biochemical and pathogenic properties of Shewanella algae and Shewanella putrefaciens publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.36.3.783-787.1998 contributor: fullname: Khashe – volume: 36 start-page: 1 year: 2015 ident: 10.1016/j.aquaculture.2016.10.038_bb0125 article-title: Functional genomics to discover antibiotic resistance genes: the paradigm of resistance to colistin mediated by ethanolamine phosphotransferase in Shewanella algae mars 14 publication-title: Int. J. Antimicrob. Agents contributor: fullname: Telke – volume: 57 start-page: 60 year: 2007 ident: 10.1016/j.aquaculture.2016.10.038_bb0145 article-title: Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov. isolated from west pacific deep-sea sediment publication-title: Int. J. Syst. Evol. Microbiol. doi: 10.1099/ijs.0.64500-0 contributor: fullname: Xiao – volume: 18 start-page: 1017 year: 2010 ident: 10.1016/j.aquaculture.2016.10.038_bb0160 article-title: Assessment of growth condition for a candidate probiotic, Shewanella algae, isolated from digestive system of a healthy juvenile Penaeus monodon publication-title: Aquac. Int. doi: 10.1007/s10499-010-9319-6 contributor: fullname: Zadeh – volume: 56 start-page: 3285 year: 1988 ident: 10.1016/j.aquaculture.2016.10.038_bb0105 article-title: Virulence properties and enterotoxin production of Aeromonas strains isolated from fish publication-title: Infect. Immun. doi: 10.1128/IAI.56.12.3285-3293.1988 contributor: fullname: Santos – volume: 41 start-page: 203 year: 2016 ident: 10.1016/j.aquaculture.2016.10.038_bb0035 article-title: Pyogenic flexor tenosynovitis caused by Shewanella algae publication-title: J. Hand. Surg. doi: 10.1016/j.jhsa.2016.04.012 contributor: fullname: Fluke – volume: 46 start-page: 264 year: 2014 ident: 10.1016/j.aquaculture.2016.10.038_bb0070 article-title: A case of spontaneous bacterial peritonitis with bacteremia caused by Shewanella algae publication-title: Infect. Chemother. doi: 10.3947/ic.2014.46.4.264 contributor: fullname: Kim – volume: 16 start-page: 1859 issue: 1845–1850 year: 2015 ident: 10.1016/j.aquaculture.2016.10.038_bb0080 article-title: Biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia publication-title: Agric. Sci. Technol. contributor: fullname: Li – volume: 74-76 year: 2012 ident: 10.1016/j.aquaculture.2016.10.038_bb0120 article-title: Isolation of Shewanella algae from pleural exudate of patient with pneumonia publication-title: Zh. Mikrobiol. Epidemiol. Immunobiol. contributor: fullname: Sivolodskiĭ – volume: 32 start-page: 79 year: 2002 ident: 10.1016/j.aquaculture.2016.10.038_bb0075 article-title: Shewanella alga bacteremia and associated cellulitis in a patient with multiple myeloma publication-title: Haematologia doi: 10.1163/156855902760262808 contributor: fullname: Krsnik – volume: 68 start-page: 263 year: 2014 ident: 10.1016/j.aquaculture.2016.10.038_bb0100 article-title: Molecular characterization and bioactivity profile of the tropical sponge-associated bacterium Shewanella algae VCDB publication-title: Helgol. Mar. Res. doi: 10.1007/s10152-014-0386-3 contributor: fullname: Rachanamol – volume: 52 start-page: 431 year: 2013 ident: 10.1016/j.aquaculture.2016.10.038_bb0085 article-title: Clinical and microbiological features of Shewanella bacteremia in patients with hepatobiliary disease publication-title: J. Intern. Med. doi: 10.2169/internalmedicine.52.8152 contributor: fullname: Liu – volume: 8 start-page: 64 year: 2015 ident: 10.1016/j.aquaculture.2016.10.038_bb0015 article-title: Neonatal sepsis caused by Shewanella algae: a case report publication-title: Australas. Med. J. doi: 10.21767/AMJ.2015.2292 contributor: fullname: Charles – volume: 47 start-page: 1 year: 2007 ident: 10.1016/j.aquaculture.2016.10.038_bb0010 article-title: Isolation, identification, phylogenetic analysis and related properties of a pathogen in Silurus meridionalis Chen publication-title: Acta Microbiol Sin. contributor: fullname: Cao – volume: 50 start-page: 304 year: 2010 ident: 10.1016/j.aquaculture.2016.10.038_bb0170 article-title: Isolation and identification of the pathogenic strain of Vibrio harveyi from Miichthys miiu publication-title: Acta Microbiol Sin. contributor: fullname: Zhang – volume: 21 start-page: 12 year: 2014 ident: 10.1016/j.aquaculture.2016.10.038_bb0055 article-title: Isolation and identification of pathogen of ascites disease from Cynoglossus semilaevis Günther and its antibiotic sensitivity analysis publication-title: J. Tianjin. Agric. Coll. contributor: fullname: Hu – volume: 17 start-page: 63 year: 2007 ident: 10.1016/j.aquaculture.2016.10.038_bb0155 article-title: Choice of optimal culture medium on Shewanella alga publication-title: Biotechnology contributor: fullname: Yue – volume: 332 start-page: 245 year: 2015 ident: 10.1016/j.aquaculture.2016.10.038_bb0140 article-title: Estimation of dietary copper (Cu) requirement of Cynoglossus semilaevis Günther publication-title: LNEE. contributor: fullname: Wang – volume: 34 start-page: 1 year: 2003 ident: 10.1016/j.aquaculture.2016.10.038_bb0020 article-title: Identification and characterization of Shewanella algae as a novel pathogen of ulcer disease of fish Scinenops ocellata publication-title: Oceanologia. Limnologia. Sinica. contributor: fullname: Chen – volume: 40 start-page: 331 year: 1990 ident: 10.1016/j.aquaculture.2016.10.038_bb0115 article-title: Taxonomy of four marine bacterial strains that produce tetrodotoxin publication-title: Int. J. Syst. Bacteriol. doi: 10.1099/00207713-40-4-331 contributor: fullname: Simidu – volume: 36 start-page: 625 year: 2015 ident: 10.1016/j.aquaculture.2016.10.038_bb0030 article-title: A case report of intracranial infection caused by Shewanella putrefaciens publication-title: Neurol. Sci. doi: 10.1007/s10072-014-1956-5 contributor: fullname: Duan – volume: 36 start-page: 764 year: 2012 ident: 10.1016/j.aquaculture.2016.10.038_bb0025 article-title: Study on pathogen of skin ulcer disease of half-smooth tongue sole (Cynoglossus semilaevis) publication-title: J. Fish. China doi: 10.3724/SP.J.1231.2012.27667 contributor: fullname: Chen – volume: 65 start-page: 3896 year: 1999 ident: 10.1016/j.aquaculture.2016.10.038_bb0040 article-title: Occurrence of Shewanella algae in Danish coastal water and effects of water temperature and culture conditions on its survival publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.65.9.3896-3900.1999 contributor: fullname: Gram – volume: 31 start-page: 112 year: 2009 ident: 10.1016/j.aquaculture.2016.10.038_bb0165 article-title: Phenotypic and molecular characterization of pathogenic Listonella anguillarum isolated from half-smooth tongue sole Cynoglossus semilaevis publication-title: Acta Oceanol. Sin. contributor: fullname: Zhang – volume: 11 start-page: 347 year: 2005 ident: 10.1016/j.aquaculture.2016.10.038_bb0050 article-title: Shewanella algae and Shewanella putrefaciens: clinical and microbiological characteristics publication-title: Clin. Microbiol. Infect. doi: 10.1111/j.1469-0691.2005.01108.x contributor: fullname: Holt |
SSID | ssj0006651 |
Score | 2.4686425 |
Snippet | In this study, a Gram-negative bacterium was isolated from diseased Cynoglossus semilaevis (C. semilaevis) and tentatively named strain CSG-15. It was... |
SourceID | crossref elsevier |
SourceType | Aggregation Database Publisher |
StartPage | 356 |
SubjectTerms | 16S rDNA Antimicrobial susceptibility Cynoglossus semilaevis Shewanella algae |
Title | Isolation, identification and characterization of Shewanella algae from reared tongue sole, Cynoglossus semilaevis Günther |
URI | https://dx.doi.org/10.1016/j.aquaculture.2016.10.038 |
Volume | 468 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1NT9tAEB1BkFB7QBRaQQvRInHESbzebLYSlygCklZwAaTcrPXuOE0FDg2xqqoSv4wbf4wZf_AhIfXQo-3d1eppPDO23nsLsC-VM47_3LuOxEAZjQG7jgXWJ6g0RjIqLIVOz_TwUn0bd8dLMKi1MEyrrHJ_mdOLbF3daVdotm-mU9b40qcFrU4dBZ8eMV6GFSpHSjVgpT_6Pjx7Sshad8uD85QKeMIq7D3TvOyv3JYmF2yaGeoWc71YrfJWmXpReo7XYa3qGUW_3NYHWMJsA973J_PKNwM34e-IYqgA-UBMfcUAKq6FzbxwT7bMpepSzFJx_gN_Wya5WMF6DhSsNBFz9rT1gjrCSY6CFsUDMfiTzSZXtM38Vtzi9fTKsiRdnDzcZ9w-foTL46OLwTCozlUIXCTDRaBT7VUvDa2lsoWEmDRWdXseDTobdZNOErLiVbuOJ2y9MS79mnpM6H31ElUUfYJGNstwC4TxqUww7aUGlVLO2iS0UY-GalSGOottkDWM8U1pnxHXvLKf8QvsY8aeHxH223BYAx6_ioWY0vy_p3_-v-lf4J3k0l0ws3egsZjnuEuNxyJpwnLrLmxW4fUIeBveKg |
link.rule.ids | 315,786,790,4521,24144,27955,27956,45618,45712 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1NSwMxEB2qgh8H8RO_jeDR1W42TSN4kaJWbXuxQm9LNpmtFd1qbRER_GXe_GNmdrdWQfDgcXeTEB7JzGR57wVglwujDP25N0WOnlASPXId87SNUEgMeJBaCtUbsnotLlqlVgEqQy0M0Srz2J_F9DRa528OcjQPHjod0vi6o4Ub3VUUdHtEawwmqBogXtf-24jnIWUpuzZPCI-aT8LOiOSlHwc6s7ggy0xf7hPTi7QqvyWpb4nndA5m84qRHWeTmocCJgswc9zu5a4ZuAiv524FpRDvsY7N-T_pM9OJZebLlDnTXLJuzK5u8FkTxUUzUnMgI50J65GjrWWuHmwPkLlBcY9VXpJu-85Nc_DEnvC-c6dJkM7OPt4TKh6X4Pr0pFmpevmtCp4JuN_3ZCytKMe-1i5poTtPcaVFqWxRodFBKSpGPuldpSlah6xVysSHscXI7VbLUQTBMown3QRXgCkb8wjjcqxQCGG0jnwdlF1TiUK5umIV-BDG8CEzzwiHrLLb8Bv2IWFPnxz2q3A0BDz8sRJCF-T_7r72v-7bMFVt1mth7bxxuQ7TnJJ4ytHegPF-b4CbrgTpR1vpEvsEeere_w |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Isolation%2C+identification+and+characterization+of+Shewanella+algae+from+reared+tongue+sole%2C+Cynoglossus+semilaevis+G%C3%BCnther&rft.jtitle=Aquaculture&rft.au=Han%2C+Zhuoran&rft.au=Sun%2C+Jingfeng&rft.au=Lv%2C+Aijun&rft.au=Sung%2C+YeongYik&rft.date=2017-02-01&rft.issn=0044-8486&rft.volume=468&rft.spage=356&rft.epage=362&rft_id=info:doi/10.1016%2Fj.aquaculture.2016.10.038&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_aquaculture_2016_10_038 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0044-8486&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0044-8486&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0044-8486&client=summon |