Monoclonal antibodies to the extracellular domain of HIV-1IIIB gp160 that neutralize infectivity, block binding to CD4, and react with diverse isolates

• Published in: AIDS research and human retroviruses Vol. 8; no. 11; p. 1875
• Main Authors: Nakamura, G R, Byrn, R, Rosenthal, K, Porter, J P, Hobbs, M R, Riddle, L, Eastman, D J, Dowbenko, D, Gregory, T, Fendly, B M
• Format: Journal Article
• Language: English
• Published: United States 01.11.1992
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; CD4 Antigens - immunology; CHO Cells; Cricetinae; Cross Reactions; Epitopes - immunology; Gene Products, env - immunology; Genetic Variation; HIV Envelope Protein gp120 - immunology; HIV Envelope Protein gp160; Neutralization Tests; Protein Conformation; Protein Precursors - immunology; Recombinant Proteins; Species Specificity; Structure-Activity Relationship

Ten monoclonal antibodies prepared against a soluble, recombinant form of gp160, derived from the IIIB isolate of HIV-1, were characterized. Four of the antibodies neutralized HIV-1IIIB infectivity in vitro, three blocked the binding of recombinant gp120 to CD4, three were reactive with gp41, and on...