Gas-sensing array application for on-line monitoring in a heat-responsive bioprocess of Streptomyces griseus HUT 6037

• Published in: Food science and biotechnology Vol. 24; no. 3; pp. 875 - 881
• Main Authors: Jung, Ho-Sup, Park, Kyung-Min, Kang, Do Hyun, Kwak, Moon Kyu, Lim, Seokwon, Chang, Pahn-Shick, Kim, Keesung
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.06.2015; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; Food Science; Nutrition; Research Article; 식품과학; gas-sensing; chitosanase; principal component analysis; heat-responsive
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-015-0113-0

The stress-responsive bioprocess concept has been developed into an environmentally friendly biosensor with low energy consumption and high recovery yield. To improve chitosanase production during Streptomyces griseus cultivation, heat-stress was applied to the bioprocess with monitoring using an on-line gas sensing system. When exposed to heat-stress with liposomes, the chitosanase productivity was 2.6 times greater than for conventional cultivation. The mixed gas components could be distinguished using a principal component analysis during chitosanase production. The online-monitoring system reflected basic changes in growth conditions and metabolite formation in cells. The array type gas-sensing system was capable of detecting bacterial infection faster than conventional sensor systems. The gas sensor system can play a key role in monitoring and controlling stress levels in a stress-responsive bioprocess.