Metabolomics analysis of Pulsatilla decoction on treatment of wetness‐heat‐induced diarrhea in rats based on UPLC–Q/TOF–MS/MS
Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on th...
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| Published in | Biomedical chromatography Vol. 33; no. 11; pp. e4629 - n/a |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.11.2019
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| Abstract | Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time‐of‐flight/mass spectrometry (UPLC–Q/TOF–MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least‐squares discriminant analysis (OPLS‐DA ) models and FC ≥1.2 or ≤0.8, 67 differential metabolites in the model and control groups and 33 differential metabolites in the model and PD groups were screened. A total of 23 differential metabolites were selected based on Venny analysis. Functional analysis showed that the differential metabolites identified were primarily involved in pentose and glucuronate interconversions, glycerophospholipid metabolism, tryptophan metabolism, starch and sucrose metabolism, and glycerolipid metabolism. This study suggested that PD exerts inhibitory effects on WHD. In particular, the significant roles of PD for treating WHD lie in regulating perturbed energy metabolism, glycerophospholipid metabolism and glycerolipid metabolism, and promoting lysoPC production restoring the function of intestinal tract. |
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| AbstractList | Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness-heat-induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry (UPLC-Q/TOF-MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least-squares discriminant analysis (OPLS-DA ) models and FC ≥1.2 or ≤0.8, 67 differential metabolites in the model and control groups and 33 differential metabolites in the model and PD groups were screened. A total of 23 differential metabolites were selected based on Venny analysis. Functional analysis showed that the differential metabolites identified were primarily involved in pentose and glucuronate interconversions, glycerophospholipid metabolism, tryptophan metabolism, starch and sucrose metabolism, and glycerolipid metabolism. This study suggested that PD exerts inhibitory effects on WHD. In particular, the significant roles of PD for treating WHD lie in regulating perturbed energy metabolism, glycerophospholipid metabolism and glycerolipid metabolism, and promoting lysoPC production restoring the function of intestinal tract. Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness-heat-induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry (UPLC-Q/TOF-MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least-squares discriminant analysis (OPLS-DA ) models and FC ≥1.2 or ≤0.8, 67 differential metabolites in the model and control groups and 33 differential metabolites in the model and PD groups were screened. A total of 23 differential metabolites were selected based on Venny analysis. Functional analysis showed that the differential metabolites identified were primarily involved in pentose and glucuronate interconversions, glycerophospholipid metabolism, tryptophan metabolism, starch and sucrose metabolism, and glycerolipid metabolism. This study suggested that PD exerts inhibitory effects on WHD. In particular, the significant roles of PD for treating WHD lie in regulating perturbed energy metabolism, glycerophospholipid metabolism and glycerolipid metabolism, and promoting lysoPC production restoring the function of intestinal tract.Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness-heat-induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry (UPLC-Q/TOF-MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least-squares discriminant analysis (OPLS-DA ) models and FC ≥1.2 or ≤0.8, 67 differential metabolites in the model and control groups and 33 differential metabolites in the model and PD groups were screened. A total of 23 differential metabolites were selected based on Venny analysis. Functional analysis showed that the differential metabolites identified were primarily involved in pentose and glucuronate interconversions, glycerophospholipid metabolism, tryptophan metabolism, starch and sucrose metabolism, and glycerolipid metabolism. This study suggested that PD exerts inhibitory effects on WHD. In particular, the significant roles of PD for treating WHD lie in regulating perturbed energy metabolism, glycerophospholipid metabolism and glycerolipid metabolism, and promoting lysoPC production restoring the function of intestinal tract. Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To investigate the therapeutic effects of PD in the treatment of WHD and elucidate the potential mechanism, we used a metabolomics strategy on the base of ultraperformance liquid chromatography coupled with quadrupole time‐of‐flight/mass spectrometry (UPLC–Q/TOF–MS/MS) and analyzed the serum samples of 32 rats to identify differential metabolites and pathways associated with the PD treatment of WHD. With variable importance for projection >1.0 in the Orthogonal partial least‐squares discriminant analysis (OPLS‐DA ) models and FC ≥1.2 or ≤0.8, 67 differential metabolites in the model and control groups and 33 differential metabolites in the model and PD groups were screened. A total of 23 differential metabolites were selected based on Venny analysis. Functional analysis showed that the differential metabolites identified were primarily involved in pentose and glucuronate interconversions, glycerophospholipid metabolism, tryptophan metabolism, starch and sucrose metabolism, and glycerolipid metabolism. This study suggested that PD exerts inhibitory effects on WHD. In particular, the significant roles of PD for treating WHD lie in regulating perturbed energy metabolism, glycerophospholipid metabolism and glycerolipid metabolism, and promoting lysoPC production restoring the function of intestinal tract. |
| Author | Ma, Qi Zhang, Xiao‐song Yao, Wan‐ling Ji, Peng Hua, Yong‐li Li, Wei Wei, Yan‐ming Peng, Xiao‐ting Hu, Jun‐jie Cheng, Xiao‐hua Jia, Ya‐qian |
| Author_xml | – sequence: 1 givenname: Yong‐li orcidid: 0000-0002-9129-2381 surname: Hua fullname: Hua, Yong‐li email: huayongli2004@163.com organization: Gansu Agricultural University – sequence: 2 givenname: Qi surname: Ma fullname: Ma, Qi organization: Southwest University – sequence: 3 givenname: Wei surname: Li fullname: Li, Wei – sequence: 4 givenname: Xiao‐song surname: Zhang fullname: Zhang, Xiao‐song organization: Gansu Agricultural University – sequence: 5 givenname: Xiao‐hua surname: Cheng fullname: Cheng, Xiao‐hua – sequence: 6 givenname: Ya‐qian surname: Jia fullname: Jia, Ya‐qian organization: Gansu Agricultural University – sequence: 7 givenname: Xiao‐ting surname: Peng fullname: Peng, Xiao‐ting organization: Gansu Agricultural University – sequence: 8 givenname: Wan‐ling surname: Yao fullname: Yao, Wan‐ling organization: Gansu Agricultural University – sequence: 9 givenname: Peng surname: Ji fullname: Ji, Peng organization: Gansu Agricultural University – sequence: 10 givenname: Jun‐jie surname: Hu fullname: Hu, Jun‐jie organization: Gansu Agricultural University – sequence: 11 givenname: Yan‐ming orcidid: 0000-0001-9632-1885 surname: Wei fullname: Wei, Yan‐ming email: weiym@gsau.edu.cn organization: Gansu Agricultural University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31242331$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_aqrep_2024_102086 crossref_primary_10_1016_j_jep_2024_117901 crossref_primary_10_3389_fcimb_2020_00319 crossref_primary_10_3389_fphar_2021_668418 crossref_primary_10_1016_j_jep_2022_115741 crossref_primary_10_1097_MD_0000000000027752 crossref_primary_10_1186_s12906_022_03766_z crossref_primary_10_3389_fphar_2023_1113213 crossref_primary_10_1186_s12906_020_02974_9 crossref_primary_10_1016_j_chmed_2025_02_002 crossref_primary_10_3389_fphar_2024_1399829 crossref_primary_10_4251_wjgo_v16_i7_3193 crossref_primary_10_3389_fphar_2020_00197 crossref_primary_10_3389_fvets_2022_703051 crossref_primary_10_1002_bmc_5239 crossref_primary_10_1186_s12906_023_04244_w |
| Cites_doi | 10.1053/j.gastro.2009.08.041 10.1016/j.ehmc.2012.12.002 10.1016/j.livres.2018.09.008 10.1016/j.jep.2017.03.030 10.1016/j.eujim.2018.02.007 10.3389/fphar.2018.00053 10.1016/j.jpba.2013.03.028 10.1016/j.phrs.2018.04.009 10.1016/j.jpba.2016.11.021 10.1038/s41467-019-09329-0 10.1016/j.cbi.2014.02.014 10.1038/srep17068 10.3109/1354750X.2012.724450 10.1016/j.mam.2017.06.002 10.1016/j.jsbmb.2018.08.008 10.1016/j.jpba.2017.08.042 10.1016/j.biopha.2018.01.023 10.1053/j.gastro.2012.09.055 10.1016/j.jtcms.2017.08.001 10.1016/S2095-3119(14)60896-0 10.1016/S0092-8674(03)00422-7 10.5144/0256-4947.2001.283 10.1152/ajpgi.00172.2011 10.1074/jbc.M109.024869 10.1016/j.jchromb.2018.08.023 10.1016/j.ijbiomac.2017.02.060 10.1016/0016-5085(86)91126-1 10.1016/S0254-6272(12)60031-4 10.1016/j.jep.2018.05.018 10.1016/j.jchromb.2017.12.035 10.1016/j.cbi.2012.12.002 10.1021/ac051495j 10.1016/j.chroma.2016.05.056 10.1016/j.jtcms.2018.07.003 10.1016/j.cbi.2017.06.011 10.1021/pr3007792 10.1016/j.jpba.2017.07.044 10.1254/fpj.64.714 10.1016/j.cca.2018.04.022 10.1016/j.jpba.2018.11.027 10.1016/j.jchromb.2015.12.026 |
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| References | 2018; 164 2018; 483 1986; 90 2014; 215 2015; 5 2013; 2 2017; 4 2006; 78 2019; 10 2018; 223 2018; 1097–1098 2018; 147 2013; 144 2013; 201 2016; 1026 2017; 273 2012; 17 2018; 1074‐1075 2019; 185 2012; 302 2017; 134 2003; 113 2012; 32 2009; 137 2001; 21 1968; 64 2013; 81‐82 2018; 19 2018; 9 2018; 133 2018; 2 2018; 5 2013; 12 2017; 56 2014; 13 2016; 1454 2009; 284 2018; 50 2011; 27 2017; 146 2018; 98 2017; 202 2017; 105 e_1_2_8_28_1 e_1_2_8_29_1 Zhao Siyua L. Z. (e_1_2_8_38_1) 2018; 50 e_1_2_8_24_1 e_1_2_8_25_1 e_1_2_8_26_1 e_1_2_8_27_1 e_1_2_8_3_1 e_1_2_8_2_1 e_1_2_8_5_1 e_1_2_8_4_1 e_1_2_8_7_1 e_1_2_8_6_1 e_1_2_8_9_1 e_1_2_8_8_1 e_1_2_8_20_1 e_1_2_8_43_1 e_1_2_8_21_1 e_1_2_8_42_1 e_1_2_8_22_1 e_1_2_8_23_1 e_1_2_8_44_1 e_1_2_8_41_1 e_1_2_8_40_1 e_1_2_8_17_1 e_1_2_8_18_1 e_1_2_8_39_1 e_1_2_8_19_1 e_1_2_8_13_1 e_1_2_8_36_1 e_1_2_8_14_1 e_1_2_8_35_1 e_1_2_8_15_1 e_1_2_8_37_1 e_1_2_8_32_1 e_1_2_8_10_1 e_1_2_8_31_1 e_1_2_8_11_1 e_1_2_8_34_1 e_1_2_8_12_1 e_1_2_8_33_1 Lu S. W. (e_1_2_8_16_1) 2011; 27 e_1_2_8_30_1 |
| References_xml | – volume: 81‐82 start-page: 202 year: 2013 end-page: 209 article-title: Renal metabolic profiling of early renal injury and renoprotective effects of epidermis using UPLC Q‐TOF/HSMS/MSE publication-title: Journal of Pharmaceutical and Biomedical Analysis – volume: 19 start-page: 72 year: 2018 end-page: 79 article-title: Plasma metabolomics coupled with MetaboAnalyst and Ingenuity Pathway Analysis characterizes linoleic acid metabolism disorder in patients with spleen‐yang‐deficiency syndrome publication-title: European Journal of Integrative Medicine – volume: 4 start-page: 65 issue: 1 year: 2017 end-page: 70 article-title: Roles of three classic TCM formulae in inhibiting gastric cancer growth in mice publication-title: Journal of Traditional Chinese Medical Sciences – volume: 1097–1098 start-page: 128 year: 2018 end-page: 141 article-title: Mechanism of YLTZ on glycolipid metabolism based on UPLC/TOF/MS metabolomics publication-title: Journal of Chromatography B – volume: 5 year: 2015 article-title: System‐wide assembly of pathways and modules hierarchically reveal metabolic mechanism of cerebral ischemia publication-title: Scientific Reports – volume: 302 start-page: G618 issue: 16 year: 2012 end-page: G627 article-title: LPA stimulates intestinal DRA gene transcription via LPA2 receptor, PI3K/AKT, and c‐Fos‐dependent pathway publication-title: American Journal of Physiology. Gastrointestinal and Liver Physiology – volume: 284 start-page: 33833 issue: 49 year: 2009 end-page: 33840 article-title: Lysophosphatidylcholine activates adipocyte glucose uptake and lowers blood glucose levels in murine models of diabetes publication-title: Journal of Biological Chemistry – volume: 1454 start-page: 15 year: 2016 end-page: 25 article-title: Simultaneous quantification of 50 bioactive compounds of the traditional Chinese medicine formula Gegen‐Qinlian decoction using ultra‐high performance liquid chromatography coupled with tandem mass spectrometry publication-title: Journal of Chromatography. A – volume: 32 start-page: 52 issue: 1 year: 2012 end-page: 57 article-title: Effects of Wei Chang an pill on enzyme activity and levels of vasoactive peptide and substance P in the small intestine of rats with compound diarrhea publication-title: Journal of Traditional Chinese Medicine – volume: 90 start-page: 702 issue: 3 year: 1986 end-page: 709 article-title: Ursodeoxycholic acid, 7‐ketolithocholic acid, and chenodeoxycholic acid are primary bile acids of the nutria ( ) publication-title: Gastroenterology – volume: 5 start-page: 283 year: 2018 end-page: 290 article-title: Study on the regulation of brain–gut peptide by Shenling Baizhu San in functional diarrhea rats publication-title: Journal of Traditional Chinese Medical Sciences – volume: 105 start-page: 1622 issue: Pt 3 year: 2017 end-page: 1629 article-title: Structural modulation of gut microbiota during alleviation of antibiotic‐associated diarrhea with herbal formula publication-title: International Journal of Biological Macromolecules – volume: 56 start-page: 54 year: 2017 end-page: 65 article-title: Interactions between gut bacteria and bile in health and disease publication-title: Molecular Aspects of Medicine – volume: 98 start-page: 834 year: 2018 end-page: 846 article-title: Er Shen Wan extract reduces diarrhea and regulates AQP 4 and NHE 3 in a rat model of spleen‐kidney Yang deficiency‐induced diarrhea publication-title: Biomedicine & Pharmacotherapy – volume: 78 start-page: 567 issue: 2 year: 2006 end-page: 574 article-title: Large‐scale human metabolomics studies: A strategy for data (pre‐) processing and validation publication-title: Journal of Analytical Chemistry – volume: 2 start-page: 180 issue: 4 year: 2018 end-page: 185 article-title: Regulation of bile acid receptor activity publication-title: Liver Research – volume: 202 start-page: 265 year: 2017 end-page: 280 article-title: Treatment effects and mechanisms of Yujin Powder on rat model of large intestine dampness‐heat syndrome publication-title: Journal of Ethnopharmacology – volume: 12 start-page: 692 issue: 2 year: 2013 end-page: 703 article-title: Intrarenal metabolomic investigation of chronic kidney disease and its TGF‐ 1 mechanism in induced‐adenine rats using UPLC Q‐TOF/HSMS/MS(E) publication-title: Journal of Proteome Research – volume: 215 start-page: 7 year: 2014 end-page: 16 article-title: UPLC–MS(E) application in disease biomarker discovery: The discoveries in proteomics to metabolomics publication-title: Chemico‐Biological Interactions – volume: 27 start-page: 106 issue: 1 year: 2011 article-title: Molecular mechanisms involved in the treatment of inflammatory bowel disease by decoction publication-title: Chinese Journal of Applied Physiology – volume: 144 start-page: 145 issue: 1 year: 2013 end-page: 154 article-title: The receptor TGR5 mediates the prokinetic actions of intestinal bile acids and is required for normal defecation in mice publication-title: Gastroenterology – volume: 146 start-page: 206 year: 2017 end-page: 213 article-title: Permeability through the Caco‐2 cell monolayer of 42 bioactive compounds in the TCM formula Gegen‐Qinlian Decoction by liquid chromatography tandem mass spectrometry analysis publication-title: Journal of Pharmaceutical and Biomedical Analysis – volume: 185 start-page: 142 year: 2019 end-page: 149 article-title: Follicular dynamics of glycerophospholipid and sphingolipid metabolisms in polycystic ovary syndrome patients publication-title: The Journal of Steroid Biochemistry and Molecular Biology – volume: 1026 start-page: 227 year: 2016 end-page: 235 article-title: A GC–MS urinary quantitative metabolomics analysis in depressed patients treated with TCM formula of Xiaoyaosan publication-title: Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences – volume: 223 start-page: 51 year: 2018 end-page: 62 article-title: decoction inhibits proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin‐1 signaling pathway publication-title: Journal of Ethnopharmacology – volume: 273 start-page: 133 year: 2017 end-page: 141 article-title: Metabolomics highlights pharmacological bioactivity and biochemical mechanism of traditional Chinese medicine publication-title: Chemico‐Biological Interactions – volume: 64 start-page: 714 issue: 6 year: 1968 end-page: 729 article-title: Pharmacological studies on the components of Blume publication-title: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica – volume: 147 start-page: 149 year: 2018 end-page: 173 article-title: Quality assurance procedures for mass spectrometry untargeted metabolomics. a review publication-title: Journal of Pharmaceutical and Biomedical Analysis – volume: 134 start-page: 43 year: 2017 end-page: 52 article-title: Simultaneous determination of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin in rat plasma by UPLC–ESI–MS/MS and its application to a comparative pharmacokinetic study in normal and ulcerative colitis rats publication-title: Journal of Pharmaceutical and Biomedical Analysis – volume: 483 start-page: 57 year: 2018 end-page: 63 article-title: Mass spectrometry‐based metabolomics for tuberculosis meningitis publication-title: Clinica Chimica Acta – volume: 21 start-page: 283 issue: 5–6 year: 2001 end-page: 286 article-title: Levels of cholesteryl esters and other lipids in the plasma of patients with end‐stage renal failure publication-title: Annals of Saudi Medicine – volume: 164 start-page: 598 year: 2018 end-page: 606 article-title: Design of experiments in metabolomics‐related studies: An overview publication-title: Journal of Pharmaceutical and Biomedical Analysis – volume: 201 start-page: 31 issue: 1–3 year: 2013 end-page: 38 article-title: UPLC–Q‐TOF/HSMS/MS(E)‐based metabonomics for adenine‐induced changes in metabolic profiles of rat faeces and intervention effects of ergosta‐4,6,8(14),22‐tetraen‐3‐one publication-title: Chemico‐Biological Interactions – volume: 133 start-page: 170 year: 2018 end-page: 186 article-title: Microbiome‐mediated bile acid modification: Role in intestinal drug absorption and metabolism publication-title: Pharmacological Research – volume: 9 year: 2018 article-title: An integrated lipidomics and phenotype study reveals protective effect and biochemical mechanism of traditionally used Juzepzuk in chronic kidney disease publication-title: Frontiers in Pharmacology – volume: 137 start-page: 1649 issue: 5 year: 2009 end-page: 1660 article-title: Serotonin has a key role in pathogenesis of experimental colitis publication-title: Gastroenterology – volume: 1074‐1075 start-page: 51 year: 2018 end-page: 60 article-title: Urinary metabolomics study the mechanism of Taohong Siwu Decoction intervention in acute blood stasis model rats based on liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry publication-title: Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences – volume: 50 start-page: 12 year: 2018 end-page: 72 article-title: Anti‐inflammatory effects of Zhishi and Zhiqiao revealed by network pharmacology integrated with molecular mechanism and metabolomics studies publication-title: Phytomedicine – volume: 17 start-page: 721 issue: 8 year: 2012 end-page: 729 article-title: Application of faecal metabonomics on an experimental model of tubulointerstitial fibrosis by ultra performance liquid chromatography/high‐sensitivity mass spectrometry with MS(E) data collection technique publication-title: Biomarkers – volume: 10 start-page: 1476 issue: 1 year: 2019 article-title: Identification of serum metabolites associating with chronic kidney disease progression and anti‐fibrotic effect of 5‐methoxytryptophan publication-title: Nature Communications – volume: 2 issue: 2 year: 2013 end-page: e246 article-title: Diarrhea publication-title: Hospital Medicine Clinics – volume: 113 start-page: 717 issue: 6 year: 2003 end-page: 730 article-title: Apoptotic cells induce migration of phagocytes via caspase‐3‐mediated release of a lipid attraction signal publication-title: Cell – volume: 13 start-page: 2558 issue: 11 year: 2014 end-page: 2561 article-title: Effects of polysaccharides from decoction on the microvascular endothelial glycocalyx publication-title: Journal of Integrative Agriculture – ident: e_1_2_8_7_1 doi: 10.1053/j.gastro.2009.08.041 – ident: e_1_2_8_9_1 doi: 10.1016/j.ehmc.2012.12.002 – ident: e_1_2_8_23_1 doi: 10.1016/j.livres.2018.09.008 – ident: e_1_2_8_31_1 doi: 10.1016/j.jep.2017.03.030 – ident: e_1_2_8_13_1 doi: 10.1016/j.eujim.2018.02.007 – ident: e_1_2_8_4_1 doi: 10.3389/fphar.2018.00053 – ident: e_1_2_8_40_1 doi: 10.1016/j.jpba.2013.03.028 – ident: e_1_2_8_6_1 doi: 10.1016/j.phrs.2018.04.009 – ident: e_1_2_8_29_1 doi: 10.1016/j.jpba.2016.11.021 – ident: e_1_2_8_3_1 doi: 10.1038/s41467-019-09329-0 – ident: e_1_2_8_41_1 doi: 10.1016/j.cbi.2014.02.014 – ident: e_1_2_8_43_1 doi: 10.1038/srep17068 – ident: e_1_2_8_39_1 doi: 10.3109/1354750X.2012.724450 – ident: e_1_2_8_15_1 doi: 10.1016/j.mam.2017.06.002 – ident: e_1_2_8_14_1 doi: 10.1016/j.jsbmb.2018.08.008 – ident: e_1_2_8_25_1 doi: 10.1016/j.jpba.2017.08.042 – ident: e_1_2_8_27_1 doi: 10.1016/j.biopha.2018.01.023 – ident: e_1_2_8_2_1 doi: 10.1053/j.gastro.2012.09.055 – ident: e_1_2_8_34_1 doi: 10.1016/j.jtcms.2017.08.001 – ident: e_1_2_8_36_1 doi: 10.1016/S2095-3119(14)60896-0 – ident: e_1_2_8_11_1 doi: 10.1016/S0092-8674(03)00422-7 – ident: e_1_2_8_8_1 doi: 10.5144/0256-4947.2001.283 – ident: e_1_2_8_20_1 doi: 10.1152/ajpgi.00172.2011 – ident: e_1_2_8_32_1 doi: 10.1074/jbc.M109.024869 – ident: e_1_2_8_44_1 doi: 10.1016/j.jchromb.2018.08.023 – ident: e_1_2_8_17_1 doi: 10.1016/j.ijbiomac.2017.02.060 – ident: e_1_2_8_22_1 doi: 10.1016/0016-5085(86)91126-1 – ident: e_1_2_8_18_1 doi: 10.1016/S0254-6272(12)60031-4 – ident: e_1_2_8_30_1 doi: 10.1016/j.jep.2018.05.018 – ident: e_1_2_8_37_1 doi: 10.1016/j.jchromb.2017.12.035 – ident: e_1_2_8_42_1 doi: 10.1016/j.cbi.2012.12.002 – ident: e_1_2_8_19_1 doi: 10.1021/ac051495j – ident: e_1_2_8_26_1 doi: 10.1016/j.chroma.2016.05.056 – ident: e_1_2_8_12_1 doi: 10.1016/j.jtcms.2018.07.003 – ident: e_1_2_8_24_1 doi: 10.1016/j.cbi.2017.06.011 – volume: 50 start-page: 12 year: 2018 ident: e_1_2_8_38_1 article-title: Anti‐inflammatory effects of Zhishi and Zhiqiao revealed by network pharmacology integrated with molecular mechanism and metabolomics studies publication-title: Phytomedicine – ident: e_1_2_8_33_1 doi: 10.1021/pr3007792 – ident: e_1_2_8_5_1 doi: 10.1016/j.jpba.2017.07.044 – volume: 27 start-page: 106 issue: 1 year: 2011 ident: e_1_2_8_16_1 article-title: Molecular mechanisms involved in the treatment of inflammatory bowel disease by Pulsatilla decoction publication-title: Chinese Journal of Applied Physiology – ident: e_1_2_8_28_1 doi: 10.1254/fpj.64.714 – ident: e_1_2_8_35_1 doi: 10.1016/j.cca.2018.04.022 – ident: e_1_2_8_10_1 doi: 10.1016/j.jpba.2018.11.027 – ident: e_1_2_8_21_1 doi: 10.1016/j.jchromb.2015.12.026 |
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| Snippet | Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To... Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness‐heat‐induced diarrhea (WHD). To... Pulsatilla decoction (PD) is a classical prescription in traditional Chinese medicine that has therapeutic effects on wetness-heat-induced diarrhea (WHD). To... |
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| SubjectTerms | Animals Chromatography, High Pressure Liquid Cytokines - blood Diarrhea - etiology Diarrhea - metabolism Drugs, Chinese Herbal - pharmacology Female Hot Temperature - adverse effects Male Metabolome - drug effects Metabolomics Pulsatilla Pulsatilla decoction Rats Rats, Wistar Reproducibility of Results Tandem Mass Spectrometry UPLC–Q/TOF–MS/MS wetness‐heat induced diarrhea |
| Title | Metabolomics analysis of Pulsatilla decoction on treatment of wetness‐heat‐induced diarrhea in rats based on UPLC–Q/TOF–MS/MS |
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