Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1766; p. 137
• Main Authors: Alonso, Sergio, Suzuki, Koichi, Yamamoto, Fumiichiro, Perucho, Manuel
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Amplified Fragment Length Polymorphism Analysis - methods; CpG Islands - drug effects; CpG Islands - genetics; DNA - chemistry; DNA - drug effects; DNA - genetics; DNA Fingerprinting; DNA Methylation; DNA Restriction Enzymes - chemistry; Epigenesis, Genetic; Genetic Loci; Genome, Human; Humans; Neoplasms - classification; Neoplasms - genetics; Oligonucleotide Array Sequence Analysis; Transcription, Genetic; DNA methylation; Epigenomics; Methylation-specific amplified fragment length polymorphism; Methylation microarray

Somatic, and in a minor scale also germ line, epigenetic aberrations are fundamental to carcinogenesis, cancer progression, and tumor phenotype. DNA methylation is the most extensively studied and arguably the best understood epigenetic mechanisms that become altered in cancer. Both somatic loss of...