Physiologic relevance of the membrane attack complex inhibitory protein CD59 in human seminal plasma: CD59 is present on extracellular organelles (prostasomes), binds cell membranes, and inhibits complement-mediated lysis

• Published in: The Journal of experimental medicine Vol. 177; no. 5; pp. 1409 - 1420
• Main Authors: Rooney, I A, Atkinson, J P, Krul, E S, Schonfeld, G, Polakoski, K, Saffitz, J E, Morgan, B P
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.05.1993; The Rockefeller University Press
• Subjects: 3T3 Cells; Animals; Antigens, CD - isolation & purification; Antigens, CD - metabolism; Antigens, CD - physiology; Antigens, CD - ultrastructure; Biological and medical sciences; CD59 Antigens; Cell Membrane - metabolism; Chromatography, Liquid; Complement; Complement Membrane Attack Complex - antagonists & inhibitors; Electrophoresis, Polyacrylamide Gel; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glycosylphosphatidylinositols - metabolism; Guinea Pigs; Humans; Male; Membrane Glycoproteins - isolation & purification; Membrane Glycoproteins - metabolism; Membrane Glycoproteins - physiology; Membrane Glycoproteins - ultrastructure; Mice; Microscopy, Electron; Molecular immunology; Organelles - metabolism; Protein Binding; Semen - chemistry; Semen - physiology; Human; Vesicle; Cytolysis; Membrane protein; Spermatozoa; Molecular interaction; Complement; Inhibitor; Seminal plasma

We demonstrate here that CD59, an inhibitor of the membrane attack complex (MAC) of the complement system, is present in cell-free seminal plasma (SP) at a concentration of at least 20 micrograms/ml. Analyses by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and Edman d...