Specific interactions of leucine with disaccharides by electrospray ionization mass spectrometry: application to rapid differentiation of disaccharide isomers in combination with statistical analysis

• Published in: Analyst (London) Vol. 140; no. 23; pp. 7965 - 7973
• Main Authors: Yuan, Hang, Ying, Jianxi, Deng, Peiran, Chen, Peng, Shi, Jinwen, Liu, Yan, Gao, Xiang, Zhao, Yufen
• Format: Journal Article
• Language: English
• Published: England 07.12.2015
• Subjects: Amino acids; Carbohydrate Sequence; Chemistry Techniques, Analytical - methods; Coordination Complexes - chemistry; Differentiation; Disaccharides - chemistry; Fragmentation; Gases; Ionization; Isomerism; Isomers; Leucine; Leucine - chemistry; Mass spectrometry; Phase Transition; Spectrometry, Mass, Electrospray Ionization; Statistical analysis

The identification of carbohydrate isomers, including mono units, linkage positions and anomeric configurations, remains an arduous subject. In this study, the natural amino acid leucine (Leu) was found to specifically interact with cellobiose (Cello) to form a series of potassium adducts as [Cello...