Post-transcriptional regulation of insulin-like growth factor binding protein-2 mRNA in diabetic rat liver

• Published in: Biochemical and biophysical research communications Vol. 189; no. 2; pp. 1031 - 1037
• Main Authors: Ooi, Guck T., Y.-H. Tseng, Lucy, Rechler, Matthew M.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.12.1992; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; ARN MENSAJERO; ARN MESSAGER; Binding and carrier proteins; Biological and medical sciences; Blotting, Northern; Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Cell Nucleus - physiology; DIABETE; DIABETES; Diabetes Mellitus, Experimental - metabolism; EXPERIMENTATION; EXPERIMENTOS; FOIE; Fundamental and applied biological sciences. Psychology; GENE; GENES; HIGADO; Insulin - pharmacology; Insulin, Isophane - pharmacology; Insulin-Like Growth Factor Binding Protein 1; Insulin-Like Growth Factor Binding Protein 2; INSULINA; INSULINE; Kinetics; Liver - drug effects; Liver - metabolism; Male; PROTEINAS; PROTEINE; Proteins; RAT; RATA; Rats; Rats, Sprague-Dawley; RNA Processing, Post-Transcriptional; RNA, Messenger - genetics; RNA, Messenger - metabolism; Somatomedins - metabolism; Transcription, Genetic - drug effects; Endocrinopathy; Rat; Diabetes mellitus; Liver; Rodentia; Insulin like growth factor 2; Insulin; Posttranscriptional modification; Protein hormone; Binding protein; Vertebrata; Chronic; Mammalia; Polypeptide; Kinetics; Growth factor
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(92)92307-J

IGFBP-1 and IGFBP-2 mRNAs are increased in the livers of streptozotocin-diabetic rats. A corresponding increase is observed in transcription of the IGFBP-1 but not the IGFBP-2 gene, indicating that the increase in steady-state levels of IGFBP-2 mRNA is a post-transcriptional effect. IGFBP-1 and IGFBP-2 mRNAs also differ in the rapidity of their response to insulin treatment: hepatic IGFBP-1 mRNA is normalized within 1 h, IGFBP-2 mRNA decreases more slowly. These differences suggest that IGFBP-2 may provide more chronic adaptation to metabolic change than IGFBP-1.