Targeted mutagenesis in the olive flounder (Paralichthys olivaceus) using the CRISPR/Cas9 system with electroporation

• Published in: Biológia Vol. 76; no. 4; pp. 1297 - 1304
• Main Authors: Wang, Ling, Tan, Xungang, Wu, Zhihao, Wang, Lijuan, Jiao, Shuang, Zou, Yuxia, Ji, Guanglei, You, Feng
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.04.2021; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cell Biology; Chorion; CRISPR; Editing; Eggs; Electroporation; Embryos; Fish; Fish eggs; Gene deletion; Gene targeting; Genes; Genome editing; Genomes; gRNA; Larvae; Life Sciences; Marine fish; Microbiology; Microinjection; mRNA; Mutation; Original Article; Paralichthys olivaceus; Plant Sciences; Site-directed mutagenesis; Survival; Zoology; Electroporation; Olive flounder; ); Genome editing; CRISPR/Cas9 system; and

As a new breeding technology, genome editing becomes a powerful tool owing to its high efficiency of gene targeting. In CRISPR/Cas9 system, how to efficiently transfer gRNA and Cas9 mRNA into embryos is an important step. Though microinjection is the most common method for operating on fish embryos,...