Physical mapping between the S and HLA-E genes in the human MHC class I region: construction of a BAC, PAC, and cosmid contig

• Published in: Immunogenetics (New York) Vol. 48; no. 6; pp. 402 - 407
• Main Authors: Shiina, T, Kikkawa, E, Saito, W, Tamiya, G, Oka, A, Watanabe, K, Yamazaki, M, Tashiro, H, Okumura, K, Ando, A, Kimura, M, Soeda, E, Pontarotti, P, Inoko, H
• Format: Journal Article
• Language: English
• Published: United States 01.11.1998
• Subjects: bacterial artificial chromosomes; Base Sequence; chromosome 6; Chromosomes, Human, Pair 6; Contig Mapping; Cosmids; DNA Primers; Genes, MHC Class I; Histocompatibility Antigens Class I - genetics; histocompatibility locus HLA; HLA Antigens - genetics; HLA-E Antigens; Humans; Male; Molecular Sequence Data; Polymerase Chain Reaction; Recombination, Genetic; Sequence Tagged Sites; yeast artificial chromosomes
• ISSN: 0093-7711; 1432-1211
• DOI: 10.1007/s002510050451

The HLA class I region on human chromosome 6p21.3 encompasses approximately 2.0 Mb from the MICB to HLA-F genes and has been covered largely with over-lapping YAC clones. In this region, the 450 kilobase (kb) segment between the MICB and S genes and 350 kb between HLA-E and HLA-92/L were subjected to nucleotide sequence determination using mainly cosmid contigs derived from cloned YACs, and were extensively characterized by our group. The S-HLA-E segment between them, however, has remained uncharacterized, because all the YAC clones containing this segment are chimeric. Thus this segment probably includes an unusual sequence which is hard to clone and propagate in yeast cells, and/or which facilitates intergenic recombination in them.