The diversity of comammox bacteria and the effect of sewage discharge on their abundance in eutrophic lake sediments

• Published in: Journal of soils and sediments Vol. 20; no. 5; pp. 2495 - 2503
• Main Authors: Xu, Yangfan, Liu, Guanglong, Hua, Yumei, Wan, Xiaoqiong, Hu, Jinlong, Zhu, Duanwei, Zhao, Jianwei
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2020; Springer Nature B.V
• Subjects: Abundance; Ammonia; Ammonia-oxidizing bacteria; AmoA gene; Aquatic plants; Archaea; Area; Bacteria; Candidatus Nitrospira inopinata; Candidatus Nitrospira nitrosa; Chestnut; Discharge; Earth and Environmental Science; Environment; Environmental Physics; Eutrophic environments; Eutrophic lakes; Eutrophic waters; Eutrophication; Household wastes; Lake deposits; Lake sediments; Natural environment; Nelumbo; Nitrification; Oxidation; Sec 2 • Physical and Biogeochemical Processes • Research Article; Sediment; Sediments; Sewage; Sewage bacteria; Sewage disposal; Sewerage; Soil Science & Conservation; Survival; Trapa natans; Rhizosphere; Sediment; Ammonia oxidizer; Comammox; Eutrophication
• DOI: 10.1007/s11368-020-02618-y
• ISSN: 1439-0108

Purpose Complete ammonia oxidation (comammox) bacteria can independently oxidize ammonia to nitrate, and their discovery has changed the long-term understanding of nitrification. Comammox bacteria have been found in a variety of natural environments, but there is still a lack of understanding regarding their presence in eutrophic lakes. The main purpose of this study was to investigate the diversity and abundance of comammox bacteria in a eutrophic lake and the effects of sewage discharge on bacterial abundance. Materials and methods Samples were taken from five areas of Lake Tangxun, China, i.e., a water chestnut ( Trapa natans ) area, a lotus ( Nelumbo ) area, a bare sediment area, a food sewage disposal area, and a domestic sewage disposal area. The diversity of comammox bacteria and the abundance of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), comammox bacteria, and anaerobic ammonia-oxidizing (anammox) bacteria were measured. Results and discussion Comammox bacteria were widely found in Lake Tangxun and were closely related to Candidatus Nitrospira nitrosa and Candidatus Nitrospira inopinata . The abundance of the comammox amoA gene was lower in the two sewage discharge areas (1.3 × 10 7 copies g −1 ) than in the emergent plant areas (1.75 × 10 8 copies g −1 ) and the bare sediment area (1.0 × 10 8 copies g −1 ). The abundance of the comammox amoA gene at the five sampling areas was higher than that of the AOA amoA gene and AOB amoA gene, which indicated that comammox bacteria had a growth advantage in this eutrophic lake. Conclusions Comammox bacteria were found in eutrophic lake sediments, and these bacteria belonged to comammox clade A. The abundance of comammox bacteria in the two sewage discharge areas was the lowest, indicating that they could survive in eutrophic waters, but eutrophication inhibited their growth.