Developing recombinant antibodies for biomarker detection

• Published in: Cancer biomarkers : section A of Disease markers Vol. 6; no. 5-6; pp. 271 - 279
• Main Authors: Baird, Cheryl L, Fischer, Christopher J, Pefaur, Noah B, Miller, Keith D, Kagan, Jacob, Srivastava, Sudhir, Rodland, Karin D
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.2010
• Subjects: Antibodies, Monoclonal - biosynthesis; Biomarkers, Tumor - analysis; Biomarkers, Tumor - immunology; Humans; Neoplasms - diagnosis; Neoplasms - immunology; Peptide Library; Proteomics - methods

Monoclonal antibodies (mAbs) have an essential role in biomarker validation and diagnostic assays. A barrier to pursuing these applications is the reliance on immunization and hybridomas to produce mAbs, which is time-consuming and may not yield the desired mAb. We recommend a process flow for affin...