Flavonoid metabolites in urine after oral administration of aqueous extract of Persea americana to rats

In the present study, urinary excretion of the flavonoid metabolites of Persea americana was investigated. Aqueous extract of the leaves of P. americana was administered by gastric probe to Sprague–Dawley rats and urine samples were collected daily for five days. The urine samples were analyzed by r...

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Bibliographic Details
Published inJournal of natural medicines Vol. 61; no. 2; pp. 200 - 204
Main Authors Owolabi, Mbang A, Coker, Herbert A. B, Jaja, Smith I
Format Journal Article
LanguageEnglish
Published Springer-Verlag 01.01.2007
Subjects
benzoic acid
excretion
flavonoids
glycosides
high performance liquid chromatography
hippuric acid
leaf extracts
metabolites
oral administration
Persea americana
propionic acid
rats
reference standards
urine
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Summary:In the present study, urinary excretion of the flavonoid metabolites of Persea americana was investigated. Aqueous extract of the leaves of P. americana was administered by gastric probe to Sprague–Dawley rats and urine samples were collected daily for five days. The urine samples were analyzed by reversed-phase HPLC–UV detection. The result obtained showed extensive degradation of the flavonoid of P. americana within the first day. The phenylalkyl acids detected, 3,4-dihydroxyphenylacetic acid (I), hippuric acid (II), 3-hydroxyphenylacetic acid (III), 3-methoxy-4-hydroxyphenylacetic acid (IV), 3-(4-hydroxyphenyl)propionic acid (V) and benzoic acid (VI) were confirmed by their HPLC retention times or by cochromatography with reference standards. No flavonoid glycosides or aglycones were detected in the urine samples.
Bibliography:http://dx.doi.org/10.1007/s11418-006-0122-7
ISSN:1340-3443
1861-0293
DOI:10.1007/s11418-006-0122-7