A selective CuII complex with 4-fluorophenoxyacetic acid hydrazide and phenanthroline displays DNA-cleaving and pro-apoptotic properties in cancer cells

• Published in: Scientific reports Vol. 11; no. 1; pp. 1 - 15
• Main Authors: Machado, Pedro Henrique Alves, Paixão, Drielly Aparecida, Lino, Ricardo Campos, de Souza, Tiago Rodrigues, de Souza Bontempo, Nayara Júnia, Sousa, Luana Munique, Van Petten de Vasconcelos Azevedo, Fernanda, Orsolin, Priscila Capelari, Lima, Paula Marynella Alves Pereira, Martins, Isabella Castro, da Costa Guerra, Joyce Ferreira, Teixeira, Samuel Cota, Araújo, Thaise Gonçalves, Goulart, Luiz Ricardo, Morelli, Sandra, Guerra, Wendell, de Oliveira Júnior, Robson José
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 27.12.2021; Nature Publishing Group UK; Nature Portfolio
• Subjects: Acids; Antitumor activity; Apoptosis; Autophagy; Biological properties; Cancer therapies; Chemotherapy; Cytotoxicity; Deoxyribonucleic acid; DNA; DNA damage; Kinases; Ligands; Melanoma; Phagocytosis; Sarcoma; Tumor cells
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-03909-1

Abstract The thin line between efficacy and toxicity has challenged cancer therapy. As copper is an essential micronutrient and is important to tumor biology, Cu II complexes emerged as an alternative to chemotherapy; however, its biological properties need to be better understood. Thus, we report in vitro the antitumor effects of two Cu II complexes named [Cu(4-fh)(phen)(ClO 4 ) 2 ] (complex 1 ) and [Cu(4-nh)(phen)(ClO 4 ) 2 ]·H 2 O (complex 2 ), in which 4-fh = 4-fluorophenoxyacetic acid hydrazide; 4-nh = 4-nitrobenzoic hydrazide and phen = 1,10-phenanthroline. Both complexes presented cytotoxic activity against tumor cells, but only complex 1 showed significant selectivity. Complex 1 also induced DNA-damage, led to G0/G1 arrest and triggered apoptosis, which was initiated by an autophagy dysfunction. The significant in vitro selectivity and the action mechanism of complex 1 are noteworthy and reveal this prodrug as promising for anticancer therapy.